ArticlePloS one2014
Identification of the maize gravitropism gene lazy plant1 by a transposon-tagging genome resequencing strategy.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Working smarter, not harder: silencingHorticulture research · 2025Article
- BRXL4-LAZY1 interaction at the plasma membrane controls Arabidopsis branch angle and gravitropism.The Plant journal : for cell and molecular biology · 2023Article
- Gravity sensing and responses in the coordination of the shoot gravitropic setpoint angle.The New phytologist · 2022Review
- Characterization of a Novel Creeping Tartary Buckwheat (Frontiers in plant science · 2022Article
- Developmental genetics of maize vegetative shoot architecture.Molecular breeding : new strategies in plant improvement · 2021Article
- Article
- LAZY3 plays a pivotal role in positive root gravitropism in Lotus japonicus.Journal of experimental botany · 2020Article
- Review
- Establishment ofBreeding science · 2019Article
- Towards the identification of a gene for prostrate tillers in barley (Hordeum vulgare L.).PloS one · 2018Article
- LAZY Genes Mediate the Effects of Gravity on Auxin Gradients and Plant Architecture.Plant physiology · 2017Article
- Useful parasites: the evolutionary biology and biotechnology applications of transposable elements.Journal of genetics · 2016Review
- Control of sexuality by theScience advances · 2016Article
- Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Since their initial discovery, transposons have been widely used as mutagens for forward and reverse genetic screens in a range of organisms. The problems of high copy number and sequence divergence among related transposons have often limited the efficiency at which tagged genes can be identified. A method was developed to identity the locations of Mutator (Mu) transposons in the Zea mays genome using a simple enrichment method combined with genome resequencing to identify transposon junction fragments. The sequencing library was prepared from genomic DNA by digesting with a restriction enzyme that cuts within a perfectly conserved motif of the Mu terminal inverted repeats (TIR). Paired-end reads containing Mu TIR sequences were computationally identified and chromosomal sequences flanking the transposon were mapped to the maize reference genome. This method has been used to identify Mu insertions in a number of alleles and to isolate the previously unidentified lazy plant1 (la1) gene. The la1 gene is required for the negatively gravitropic response of shoots and mutant plants lack the ability to sense gravity. Using bioinformatic and fluorescence microscopy approaches, we show that the la1 gene encodes a cell membrane and nuclear localized protein. Our Mu-Taq method is readily adaptable to identify the genomic locations of any insertion of a known sequence in any organism using any sequencing platform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.