ArticlePloS one2014
Biological roles of the O-methyl phosphoramidate capsule modification in Campylobacter jejuni.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- Evolution of a large periplasmic disk in Campylobacterota flagella enables both efficient motility and autoagglutination.Developmental cell · 2024Article
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- Discovery and Functional Characterization of a Clandestine ATP-Dependent Amidoligase in the Biosynthesis of the Capsular Polysaccharide fromBiochemistry · 2022Article
- Functional Characterization of Two PLP-Dependent Enzymes Involved in Capsular Polysaccharide Biosynthesis fromBiochemistry · 2021Article
- Functional and Structural Characterization of the UDP-Glucose Dehydrogenase Involved in Capsular Polysaccharide Biosynthesis fromBiochemistry · 2021Article
- Application of Bacteriophages to LimitFrontiers in microbiology · 2021Review
- Comparison of the Pathogenic Potential ofPathogens (Basel, Switzerland) · 2020Article
- Distribution of Capsular Types ofThe American journal of tropical medicine and hygiene · 2019Article
- Manganese-Induced Substrate Promiscuity in the Reaction Catalyzed by Phosphoglutamine Cytidylyltransferase from Campylobacter jejuni.Biochemistry · 2019Article
- Bacterial ABNature communications · 2019Article
- Natural Products Containing 'Rare' Organophosphorus Functional Groups.Molecules (Basel, Switzerland) · 2019Review
- Update on Campylobacter vaccine development.Human vaccines & immunotherapeutics · 2019Review
- Evaluation of a conjugate vaccine platform against enterotoxigenic Escherichia coli (ETEC), Campylobacter jejuni and Shigella.Vaccine · 2018Article
- Campylobacter jejuni: collective components promoting a successful enteric lifestyle.Nature reviews. Microbiology · 2018Review
- CapC, a Novel Autotransporter and Virulence Factor of Campylobacter jejuni.Applied and environmental microbiology · 2018Article
- Phase-Variable Genotypes Sweetened by Glycosylation Phenotypes.Journal of bacteriology · 2018Article
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12 authors.
Funding
Abstract
Campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide, and the capsular polysaccharide (CPS) of this organism is required for persistence and disease. C. jejuni produces over 47 different capsular structures, including a unique O-methyl phosphoramidate (MeOPN) modification present on most C. jejuni isolates. Although the MeOPN structure is rare in nature it has structural similarity to some synthetic pesticides. In this study, we have demonstrated, by whole genome comparisons and high resolution magic angle spinning NMR, that MeOPN modifications are common to several Campylobacter species. Using MeOPN biosynthesis and transferase mutants generated in C. jejuni strain 81-176, we observed that loss of MeOPN from the cell surface correlated with increased invasion of Caco-2 epithelial cells and reduced resistance to killing by human serum. In C. jejuni, the observed serum mediated killing was determined to result primarily from activation of the classical complement pathway. The C. jejuni MeOPN transferase mutant showed similar levels of colonization relative to the wild-type in chickens, but showed a five-fold drop in colonization when co-infected with the wild-type in piglets. In Galleria mellonella waxmoth larvae, the MeOPN transferase mutant was able to kill the insects at wild-type levels. Furthermore, injection of the larvae with MeOPN-linked monosaccharides or CPS purified from the wild-type strain did not result in larval killing, indicating that MeOPN does not have inherent insecticidal activity.
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