ArticleThe Journal of biological chemistry2014
Candida albicans utilizes a modified β-oxidation pathway for the degradation of toxic propionyl-CoA.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 60 citations in OpenAlex.
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- Proteomic Analysis gives Novel Insights into Candida Albicans Morphogenesis in the Wake of Ubiquitin Mutation UbEP42.Cell biochemistry and biophysics · 2026Article
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- The macroevolution of filamentation morphology across the Saccharomycotina yeast subphylum.FEMS yeast research · 2026Article
- Transcriptomics-based analysis of acetate and propionate transport and metabolism in Yarrowia lipolytica.Biotechnology for biofuels and bioproducts · 2025Article
- A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone onBiofilm · 2025Article
- Peroxisomes regulate virulence and cell density sensing inbioRxiv : the preprint server for biology · 2025Article
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- Metabolic pathway analysis of the methylcitrate cycle in bacteria and fungi identifies methylcitrate synthase as an antiinfective drug target.microLife · 2025Article
- Propionate metabolism inFrontiers in microbiology · 2025Article
- Central Carbon Metabolism inJournal of fungi (Basel, Switzerland) · 2024Article
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- Creation of Environmentally Friendly Super "Dinitrotoluene Scavenger" Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- The Methylcitrate Cycle and Its Crosstalk with the Glyoxylate Cycle and Tricarboxylic Acid Cycle in Pathogenic Fungi.Molecules (Basel, Switzerland) · 2023Review
- Metabolic engineering to improve production of 3-hydroxypropionic acid from corn-stover hydrolysate in Aspergillus species.Biotechnology for biofuels and bioproducts · 2023Article
- Effects of Light on Growth and Metabolism ofMicroorganisms · 2022Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Propionyl-CoA arises as a metabolic intermediate from the degradation of propionate, odd-chain fatty acids, and some amino acids. Thus, pathways for catabolism of this intermediate have evolved in all kingdoms of life, preventing the accumulation of toxic propionyl-CoA concentrations. Previous studies have shown that fungi generally use the methyl citrate cycle for propionyl-CoA degradation. Here, we show that this is not the case for the pathogenic fungus Candida albicans despite its ability to use propionate and valerate as carbon sources. Comparative proteome analyses suggested the presence of a modified β-oxidation pathway with the key intermediate 3-hydroxypropionate. Gene deletion analyses confirmed that the enoyl-CoA hydratase/dehydrogenase Fox2p, the putative 3-hydroxypropionyl-CoA hydrolase Ehd3p, the 3-hydroxypropionate dehydrogenase Hpd1p, and the putative malonate semialdehyde dehydrogenase Ald6p essentially contribute to propionyl-CoA degradation and its conversion to acetyl-CoA. The function of Hpd1p was further supported by the detection of accumulating 3-hydroxypropionate in the hpd1 mutant on propionyl-CoA-generating nutrients. Substrate specificity of Hpd1p was determined from recombinant purified enzyme, which revealed a preference for 3-hydroxypropionate, although serine and 3-hydroxyisobutyrate could also serve as substrates. Finally, virulence studies in a murine sepsis model revealed attenuated virulence of the hpd1 mutant, which indicates generation of propionyl-CoA from host-provided nutrients during infection.
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