ArticleNucleic acids research2014
The transcript elongation factor SPT4/SPT5 is involved in auxin-related gene expression in Arabidopsis.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 64 citations in OpenAlex.
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- Different elongation factors distinctly modulate RNA polymerase II transcription in Arabidopsis.Nucleic acids research · 2023Article
- Transcription elongation factors OsSPT4 and OsSPT5 are essential for rice growth and development and act with APO2.Plant cell reports · 2023Article
- Contribution of the histone variant H2A.Z to expression of responsive genes in plants.Seminars in cell & developmental biology · 2023Review
- Transcription elongation factor AtSPT4-2 positively modulates salt tolerance in Arabidopsis thaliana.BMC plant biology · 2023Article
- Phosphorylation of the FACT histone chaperone subunit SPT16 affects chromatin at RNA polymerase II transcriptional start sites in Arabidopsis.Nucleic acids research · 2022Article
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- Co-Transcriptional RNA Processing in Plants: Exploring from the Perspective of Polyadenylation.International journal of molecular sciences · 2021Review
- Multifaceted roles of RNA polymerase IV in plant growth and development.Journal of experimental botany · 2020Article
- Genetic and signalling pathways of dry fruit size: targets for genome editing-based crop improvement.Plant biotechnology journal · 2020Review
- Critical Role of Transcript Cleavage in Arabidopsis RNA Polymerase II Transcriptional Elongation.The Plant cell · 2020Article
- The UAP56-Interacting Export Factors UIEF1 and UIEF2 Function in mRNA Export.Plant physiology · 2019Article
- Elongator Is Required for Root Stem Cell Maintenance by RegulatingPlant physiology · 2019Article
- ALY RNA-Binding Proteins Are Required for Nucleocytosolic mRNA Transport and Modulate Plant Growth and Development.Plant physiology · 2018Article
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9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The heterodimeric complex SPT4/SPT5 is a transcript elongation factor (TEF) that directly interacts with RNA polymerase II (RNAPII) to regulate messenger RNA synthesis in the chromatin context. We provide biochemical evidence that in Arabidopsis, SPT4 occurs in a complex with SPT5, demonstrating that the SPT4/SPT5 complex is conserved in plants. Each subunit is encoded by two genes SPT4-1/2 and SPT5-1/2. A mutant affected in the tissue-specifically expressed SPT5-1 is viable, whereas inactivation of the generally expressed SPT5-2 is homozygous lethal. RNAi-mediated downregulation of SPT4 decreases cell proliferation and causes growth reduction and developmental defects. These plants display especially auxin signalling phenotypes. Consistently, auxin-related genes, most strikingly AUX/IAA genes, are downregulated in SPT4-RNAi plants that exhibit an enhanced auxin response. In Arabidopsis nuclei, SPT5 clearly localizes to the transcriptionally active euchromatin, and essentially co-localizes with transcribing RNAPII. Typical for TEFs, SPT5 is found over the entire transcription unit of RNAPII-transcribed genes. In SPT4-RNAi plants, elevated levels of RNAPII and SPT5 are detected within transcribed regions (including those of downregulated genes), indicating transcript elongation defects in these plants. Therefore, SPT4/SPT5 acts as a TEF in Arabidopsis, regulating transcription during the elongation stage with particular impact on the expression of certain auxin-related genes.
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