ArticleDevelopment (Cambridge, England)2014
Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT).
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Alterations of the skeletal muscle nuclear proteome after acute exercise reveals a posttranscriptional influence.American journal of physiology. Cell physiology · 2025Article
- Deciphering Precise Gene Transcriptional Expression Using gwINTACT in Tomato.Frontiers in plant science · 2022Article
- In vivo tissue-specific chromatin profiling in Drosophila melanogaster using GFP-tagged nuclei.Genetics · 2021Article
- Meiocyte Isolation by INTACT and Meiotic Transcriptome Analysis inFrontiers in plant science · 2021Article
- Control of zygotic genome activation in Xenopus.Current topics in developmental biology · 2021Review
- Understanding epigenomics based on the rice model.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Transcriptomics and Proteomics Methods forCold Spring Harbor protocols · 2020Article
- Article
- Endosperm-specific transcriptome analysis by applying the INTACT system.Plant reproduction · 2019Article
- Adapting INTACT to analyse cell-type-specific transcriptomes and nucleocytoplasmic mRNA dynamics in the Arabidopsis embryo.Plant reproduction · 2019Article
- Proteomic-based approaches to cardiac development and disease.Current opinion in chemical biology · 2019Review
- Understanding molecular mechanisms of disease through spatial proteomics.Current opinion in chemical biology · 2019Review
- Article
- Nuclear Transcriptomes at High Resolution Using Retooled INTACT.Plant physiology · 2018Article
- Transcriptome dynamics revealed by a gene expression atlas of the early Arabidopsis embryo.Nature plants · 2017Article
- Biotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome.Cell reports · 2017Article
- Chromatin remodeling during in vivo neural stem cells differentiating to neurons in early Drosophila embryos.Cell death and differentiation · 2017Article
- Epigenetic dysregulation of brainstem nuclei in the pathogenesis of Alzheimer's disease: looking in the correct place at the right time?Cellular and molecular life sciences : CMLS · 2017Review
- Chromatin remodeling during the in vivo glial differentiation in early Drosophila embryos.Scientific reports · 2016Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
The proper dissection of the molecular mechanisms governing the specification and differentiation of specific cell types requires isolation of pure cell populations from heterogeneous tissues and whole organisms. Here, we describe a method for purification of nuclei from defined cell or tissue types in vertebrate embryos using INTACT (isolation of nuclei tagged in specific cell types). This method, previously developed in plants, flies and worms, utilizes in vivo tagging of the nuclear envelope with biotin and the subsequent affinity purification of the labeled nuclei. In this study we successfully purified nuclei of cardiac and skeletal muscle from Xenopus using this strategy. We went on to demonstrate the utility of this approach by coupling the INTACT approach with liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomic methodologies to profile proteins expressed in the nuclei of developing hearts. From these studies we have identified the Xenopus orthologs of 12 human proteins encoded by genes, which when mutated in human lead to congenital heart disease. Thus, by combining these technologies we are able to identify tissue-specific proteins that are expressed and required for normal vertebrate organ development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.