ArticleDevelopment (Cambridge, England)2014
Oocyte polarity requires a Bucky ball-dependent feedback amplification loop.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 71 citations in OpenAlex.
- Article
- Dynamic maternal synthesis and segregation of the germ plasm organizer, Bucky ball, in chicken oocytes and follicles.Scientific reports · 2024Article
- Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component Mios.Nature communications · 2024Article
- Sexually dimorphic dynamics of the microtubule network in medaka (Oryzias latipes) germ cells.Development (Cambridge, England) · 2024Article
- Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component, Mios.bioRxiv : the preprint server for biology · 2024Article
- Next-generation plasmids for transgenesis in zebrafish and beyond.Development (Cambridge, England) · 2023Article
- Germ Granules in Animal Oogenesis.Journal of developmental biology · 2022Review
- Identification of chicken LOC420478 as Bucky ball equivalent and potential germ plasm organizer in birds.Scientific reports · 2022Article
- A proteomics approach identifies novel resident zebrafish Balbiani body proteins Cirbpa and Cirbpb.Developmental biology · 2022Article
- Bucky Ball Is a Novel Zebrafish Vasa ATPase Activator.Biomolecules · 2021Article
- A transgenic system for targeted ablation of reproductive and maternal-effect genes.Development (Cambridge, England) · 2021Article
- Loss ofDevelopment (Cambridge, England) · 2020Article
- Exploring the Activities of RBPMS Proteins in Myocardial Biology.Pediatric cardiology · 2019Review
- The Hippo pathway effector Taz is required for cell morphogenesis and fertilization in zebrafish.Development (Cambridge, England) · 2018Article
- Functional equivalence of germ plasm organizers.PLoS genetics · 2018Article
- rbpms2 functions in Balbiani body architecture and ovary fate.PLoS genetics · 2018Article
- Slow calcium waves mediate furrow microtubule reorganization and germ plasm compaction in the early zebrafish embryo.Development (Cambridge, England) · 2018Article
- High-Sensitivity and High-Resolution In Situ Hybridization of Coding and Long Non-coding RNAs in Vertebrate Ovaries and Testes.Biological procedures online · 2018Article
- Imaging early embryonic calcium activity with GCaMP6s transgenic zebrafish.Developmental biology · 2017Article
- Coordination of cellular differentiation, polarity, mitosis and meiosis - New findings from early vertebrate oogenesis.Developmental biology · 2017Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In vertebrates, the first asymmetries are established along the animal-vegetal axis during oogenesis, but the underlying molecular mechanisms are poorly understood. Bucky ball (Buc) was identified in zebrafish as a novel vertebrate-specific regulator of oocyte polarity, acting through unknown molecular interactions. Here we show that endogenous Buc protein localizes to the Balbiani body, a conserved, asymmetric structure in oocytes that requires Buc for its formation. Asymmetric distribution of Buc in oocytes precedes Balbiani body formation, defining Buc as the earliest marker of oocyte polarity in zebrafish. Through a transgenic strategy, we determined that excess Buc disrupts polarity and results in supernumerary Balbiani bodies in a 3'UTR-dependent manner, and we identified roles for the buc introns in regulating Buc activity. Analyses of mosaic ovaries indicate that oocyte pattern determines the number of animal pole-specific micropylar cells that are associated with an egg via a close-range signal or direct cell contact. We demonstrate interactions between Buc protein and buc mRNA with two conserved RNA-binding proteins (RNAbps) that are localized to the Balbiani body: RNA binding protein with multiple splice isoforms 2 (Rbpms2) and Deleted in azoospermia-like (Dazl). Buc protein and buc mRNA interact with Rbpms2; buc and dazl mRNAs interact with Dazl protein. Cumulatively, these studies indicate that oocyte polarization depends on tight regulation of buc: Buc establishes oocyte polarity through interactions with RNAbps, initiating a feedback amplification mechanism in which Buc protein recruits RNAbps that in turn recruit buc and other RNAs to the Balbiani body.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.