ArticleDevelopment (Cambridge, England)2014
Reconstruction of phrenic neuron identity in embryonic stem cell-derived motor neurons.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 56 citations in OpenAlex.
- Mutant TDP-43 drives impairments in axonal transport and glycolysis in a mouse stem-cell-derived motor neuron model of amyotrophic lateral sclerosis (ALS).Cell death & disease · 2026Article
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- Multimodal Hox5 activity generates motor neuron diversity.Communications biology · 2024Article
- Generation of human iPSC-derived phrenic-like motor neurons to model respiratory motor neuron degeneration in ALS.Communications biology · 2024Article
- Enrichment of human embryonic stem cell-derived V3 interneurons using an Nkx2-2 gene-specific reporter.Scientific reports · 2023Article
- Efficient generation of lower induced motor neurons by coupling Ngn2 expression with developmental cues.Cell reports · 2023Article
- Catenin signaling controls phrenic motor neuron development and function during a narrow temporal window.bioRxiv : the preprint server for biology · 2023Article
- Integrative genetic and single cell RNA sequencing analysis provides new clues to the amyotrophic lateral sclerosis neurodegeneration.Frontiers in neuroscience · 2023Article
- Catenin signaling controls phrenic motor neuron development and function during a narrow temporal window.Frontiers in neural circuits · 2023Article
- Article
- Downstream bioprocessing of human pluripotent stem cell-derived therapeutics.Engineering in life sciences · 2022Review
- Targeting phosphoglycerate kinase 1 with terazosin improves motor neuron phenotypes in multiple models of amyotrophic lateral sclerosis.EBioMedicine · 2022Article
- Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.Neuron · 2022Article
- Establishing the Molecular and Functional Diversity of Spinal Motoneurons.Advances in neurobiology · 2022Article
- Optogenetic modeling of human neuromuscular circuits in Duchenne muscular dystrophy with CRISPR and pharmacological corrections.Science advances · 2021Article
- Standardized Reporter Systems for Purification and Imaging of Human Pluripotent Stem Cell-derived Motor Neurons and Other Cholinergic Cells.Neuroscience · 2020Article
- Differential abilities to engage inaccessible chromatin diversify vertebrate Hox binding patterns.Development (Cambridge, England) · 2020Article
- Genetic Basis of Aerobically Supported Voluntary Exercise: Results from a Selection Experiment with House Mice.Genetics · 2020Article
- Novel concept for the epaxial/hypaxial boundary based on neuronal development.Journal of anatomy · 2020Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Air breathing is an essential motor function for vertebrates living on land. The rhythm that drives breathing is generated within the central nervous system and relayed via specialised subsets of spinal motor neurons to muscles that regulate lung volume. In mammals, a key respiratory muscle is the diaphragm, which is innervated by motor neurons in the phrenic nucleus. Remarkably, relatively little is known about how this crucial subtype of motor neuron is generated during embryogenesis. Here, we used direct differentiation of motor neurons from mouse embryonic stem cells as a tool to identify genes that direct phrenic neuron identity. We find that three determinants, Pou3f1, Hoxa5 and Notch, act in combination to promote a phrenic neuron molecular identity. We show that Notch signalling induces Pou3f1 in developing motor neurons in vitro and in vivo. This suggests that the phrenic neuron lineage is established through a local source of Notch ligand at mid-cervical levels. Furthermore, we find that the cadherins Pcdh10, which is regulated by Pou3f1 and Hoxa5, and Cdh10, which is controlled by Pou3f1, are both mediators of like-like clustering of motor neuron cell bodies. This specific Pcdh10/Cdh10 activity might provide the means by which phrenic neurons are assembled into a distinct nucleus. Our study provides a framework for understanding how phrenic neuron identity is conferred and will help to generate this rare and inaccessible yet vital neuronal subtype directly from pluripotent stem cells, thus facilitating subsequent functional investigations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.