ArticleAmerican journal of physiology. Endocrinology and metabolism2014
Impact of embryo number and maternal undernutrition around the time of conception on insulin signaling and gluconeogenic factors and microRNAs in the liver of fetal sheep.
Article in American journal of physiology. Endocrinology and metabolism, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Fetal glucose availability: a key regulator of the metabolic, hormonal and contractility profiles of the fetal sheep heart.The Journal of physiology · 2026Article
- Maternal high-fat high-energy diet impairs surfactant maturation in the non-human primate fetal lung.International journal of obesity (2005) · 2026Article
- Moderate maternal nutrient restriction alters type II alveolar epithelial cell density in the non-human primate fetal lung.Experimental physiology · 2026Article
- Elevated cortisol concentration in preterm sheep fetuses impacts heart development.Experimental physiology · 2025Article
- Sexually dimorphic responses in androgen metabolism and signalling in the non-human primate placenta to moderate maternal undernutrition.Biology of sex differences · 2025Article
- Sex-specific effects of betamethasone on glucocorticoid and apoptotic signalling pathways in the sheep placenta.The Journal of physiology · 2025Article
- Antenatal Vitamin C differentially affects lung development in normally grown and growth restricted sheep.Pediatric research · 2025Article
- Maternal MitoQ Treatment Is Protective Against Programmed Alterations in CYP Activity Due to Antenatal Dexamethasone.Pharmaceutics · 2025Article
- Maternal high fat-high energy diet alters metabolic factors in the non-human primate fetal heart.The Journal of physiology · 2024Article
- Cardiac growth patterns and metabolism before and after birth in swine: Role of miR in proliferation, hypertrophy and metabolism.Journal of molecular and cellular cardiology plus · 2024Article
- Chronic fetal hypoxia and antenatal Vitamin C exposure differentially regulate molecular signalling in the lung of female lambs in early adulthood.Frontiers in physiology · 2024Article
- MitoQ as an antenatal antioxidant treatment improves markers of lung maturation in healthy and hypoxic pregnancy.The Journal of physiology · 2023Article
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- miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies.International journal of molecular sciences · 2021Review
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- PTEN: A Thrifty Gene That Causes Disease in Times of Plenty?Frontiers in nutrition · 2020Article
- Evidence for liver energy metabolism programming in offspring subjected to intrauterine undernutrition during midgestation.Nutrition & metabolism · 2019Article
- Epigenetic effects of the pregnancy Mediterranean diet adherence on the offspring metabolic syndrome markers.Journal of physiology and biochemistry · 2017Review
- Identification of novel microRNAs in the sheep heart and their regulation in heart failure.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
Abstract
This study aimed to determine whether exposure of the oocyte and/or embryo to maternal undernutrition results in the later programming of insulin action in the liver and factors regulating gluconeogenesis. To do this, we collect livers from singleton and twin fetal sheep that were exposed to periconceptional (PCUN; -60 to 7 days) or preimplantation (PIUN; 0-7 days) undernutrition at 136-138 days of gestation (term = 150 days). The mRNA and protein abundance of insulin signaling and gluconeogenic factors were then quantified using qRT-PCR and Western blotting, respectively, and global microRNA expression was quantified using deep sequencing methodology. We found that hepatic PEPCK-C mRNA (P < 0.01) and protein abundance and the protein abundance of IRS-1 (P < 0.01), p110β (P < 0.05), PTEN (P < 0.05), CREB (P < 0.01), and pCREB (Ser(133); P < 0.05) were decreased in the PCUN and PIUN singletons. In contrast, hepatic protein abundance of IRS-1 (P < 0.01), p85 (P < 0.01), p110β (P < 0.001), PTEN (P < 0.01), Akt2 (P < 0.01), p-Akt (Ser(473); P < 0.01), and p-FOXO-1 (Thr24) (P < 0.01) was increased in twins. There was a decrease in PEPCK-C mRNA (P < 0.01) but, paradoxically, an increase in PEPCK-C protein (P < 0.001) in twins. Both PCUN and PIUN altered the hepatic expression of 23 specific microRNAs. We propose that the differential impact of maternal undernutrition in the presence of one or two embryos on mRNAs and proteins involved in the insulin signaling and gluconeogenesis is explained by changes in the expression of a suite of specific candidate microRNAs.
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Registered trials
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