ArticleBMC plant biology2014
The C-terminal propeptide of a plant defensin confers cytoprotective and subcellular targeting functions.
Article in BMC plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Defensins of Grasses: A Systematic Review.Biomolecules · 2020Pooled it
- Genome-wide identification, characterization, and expression analysis of the plant defensin (PDF) gene family in soybean.BMC plant biology · 2026Article
- Computational insights and insecticidal activity of black gram defensin proteins against bruchids (Callosobruchus maculatus).Scientific reports · 2025Article
- Over-expression of Tfgd2-RsAFP2 fusion gene isolated from fenugreek and radish shows enhanced disease resistance against Alternaria blight disease caused by Alternaria alternata in transgenic pigeonpea.Molecular biology reports · 2025Article
- Genomic and functional insights into the diversity of3 Biotech · 2025Article
- Plant Antimicrobial Peptides and Their Main Families and Roles: A Review of the Literature.Current issues in molecular biology · 2024Review
- Characterization and functional analysis of gerberaaBIOTECH · 2024Article
- Plant-based production of an orally active cyclotide for the treatment of multiple sclerosis.Transgenic research · 2023Article
- Article
- Use of Defensins to Develop Eco-Friendly Alternatives to Synthetic Fungicides to Control Phytopathogenic Fungi and Their Mycotoxins.Journal of fungi (Basel, Switzerland) · 2022Review
- In silico prediction and mass spectrometric characterization of botanical antimicrobial peptides.Methods in enzymology · 2022Article
- Legume NCRs and nodule-specific defensins of actinorhizal plants-Do they share a common origin?PloS one · 2022Article
- Short Peptides Make a Big Difference: The Role of Botany-Derived AMPs in Disease Control and Protection of Human Health.International journal of molecular sciences · 2021Review
- Analysis of a gene family for PDF-like peptides from Arabidopsis.Scientific reports · 2021Article
- Too Hot to Handle: Antibacterial Peptides Identified in Ghost Pepper.Journal of natural products · 2021Article
- Structural Diversity and Highly Specific Host-Pathogen Transcriptional Regulation of Defensin Genes Is Revealed in Tomato.International journal of molecular sciences · 2020Article
- Plant Defensins from a Structural Perspective.International journal of molecular sciences · 2020Review
- Improving the Digestibility of Plant Defensins to Meet Regulatory Requirements for Transgene Products in Crop Protection.Frontiers in plant science · 2020Article
- Characterization, expression profiling, and functional analysis of a Populus trichocarpa defensin gene and its potential as an anti-Agrobacterium rooting medium additive.Scientific reports · 2019Article
- Plant defensins: types, mechanism of action and prospects of genetic engineering for enhanced disease resistance in plants.3 Biotech · 2019Review
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlant defensins are small (45-54 amino acids), basic, cysteine-rich proteins that have a major role in innate immunity in plants. Many defensins are potent antifungal molecules and are being evaluated for their potential to create crop plants with sustainable disease resistance. Defensins are produced as precursor molecules which are directed into the secretory pathway and are divided into two classes based on the absence (class I) or presence (class II) of an acidic C-terminal propeptide (CTPP) of about 33 amino acids. The function of this CTPP had not been defined.
resultsBy transgenically expressing the class II plant defensin NaD1 with and without its cognate CTPP we have demonstrated that NaD1 is phytotoxic to cotton plants when expressed without its CTPP. Transgenic cotton plants expressing constructs encoding the NaD1 precursor with the CTPP had the same morphology as non-transgenic plants but expression of NaD1 without the CTPP led to plants that were stunted, had crinkled leaves and were less viable. Immunofluorescence microscopy and transient expression of a green fluorescent protein (GFP)-CTPP chimera were used to confirm that the CTPP is sufficient for vacuolar targeting. Finally circular dichroism and NMR spectroscopy were used to show that the CTPP adopts a helical confirmation.
conclusionsIn this report we have described the role of the CTPP on NaD1, a class II defensin from Nicotiana alata flowers. The CTPP of NaD1 is sufficient for vacuolar targeting and plays an important role in detoxification of the defensin as it moves through the plant secretory pathway. This work may have important implications for the use of defensins for disease protection in transgenic crops.
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