ArticleJournal of the American Chemical Society2014
Engineering the assembly of heme cofactors in man-made proteins.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Controlling heme redox properties in peptide amphiphile fibers with sequence and heme loading ratio.Biophysical journal · 2024Article
- Cellular production of a de novo membrane cytochrome.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Tailorable Tetrahelical Bundles as a Toolkit for Redox Studies.The journal of physical chemistry. B · 2022Article
- De novo protein design of photochemical reaction centers.Nature communications · 2022Article
- Rational design of photosynthetic reaction center protein maquettes.Frontiers in molecular biosciences · 2022Article
- Protein-Embedded Metalloporphyrin Arrays Templated by Circularly Permuted Tobacco Mosaic Virus Coat Proteins.ACS nano · 2021Article
- Designing minimalist membrane proteins.Biochemical Society transactions · 2019Review
- Redesign of a Copper Storage Protein into an Artificial Hydrogenase.ACS catalysis · 2019Article
- A synthetic biological quantum optical system.Nanoscale · 2018Article
- Supramolecular polymer formation by aFEBS open bio · 2018Article
- De novo design of a hyperstable non-natural protein-ligand complex with sub-Å accuracy.Nature chemistry · 2017Article
- Multi-step excitation energy transfer engineered in genetic fusions of natural and synthetic light-harvesting proteins.Journal of the Royal Society, Interface · 2017Article
- The soluble loop BC region guides, but not dictates, the assembly of the transmembrane cytochrome b6.PloS one · 2017Article
- Design and engineering of water-soluble light-harvesting protein maquettes.Chemical science · 2017Article
- Strong Coupling of Localized Surface Plasmons to Excitons in Light-Harvesting Complexes.Nano letters · 2016Article
- Design and engineering of a man-made diffusive electron-transport protein.Biochimica et biophysica acta · 2016Article
- De Novo Construction of Redox Active Proteins.Methods in enzymology · 2016Article
- Engineering and Evolution of Molecular Chaperones and Protein Disaggregases with Enhanced Activity.Frontiers in molecular biosciences · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Timely ligation of one or more chemical cofactors at preselected locations in proteins is a critical preamble for catalysis in many natural enzymes, including the oxidoreductases and allied transport and signaling proteins. Likewise, ligation strategies must be directly addressed when designing oxidoreductase and molecular transport functions in man-made, first-principle protein constructs intended to operate in vitro or in vivo. As one of the most common catalytic cofactors in biology, we have chosen heme B, along with its chemical analogues, to determine the kinetics and barriers to cofactor incorporation and bishistidine ligation in a range of 4-α-helix proteins. We compare five elementary synthetic designs (maquettes) and the natural cytochrome b562 that differ in oligomeric forms, apo- and holo-tertiary structural stability; qualities that we show can either assist or hinder assembly. The cofactor itself also imposes an assembly barrier if amphiphilicity ranges toward too hydrophobic or hydrophilic. With progressive removal of identified barriers, we achieve maquette assembly rates as fast as native cytochrome b562, paving the way to in vivo assembly of man-made hemoprotein maquettes and integration of artificial proteins into enzymatic pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.