ArticleMolecular pharmaceutics2014
Engineering anisotropic biomimetic fibrocartilage microenvironment by bioprinting mesenchymal stem cells in nanoliter gel droplets.
Article in Molecular pharmaceutics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 176 citations in OpenAlex.
- Engineering anisotropic tissues: from structured scaffolds to magnetic actuation.Materials today. Bio · 2026Review
- Spheroids from Epithelial and Mesenchymal Cell Phenotypes as Building Blocks in Bioprinting (Review).Sovremennye tekhnologii v meditsine · 2025Review
- Droplet-based bioprinting.Nature reviews. Methods primers · 2025Article
- Hierarchy Reproduction: Multiphasic Strategies for Tendon/Ligament-Bone Junction Repair.Biomaterials research · 2025Review
- Automatic Photo-Cross-Linking System for Robotic-Based In Situ Bioprinting.ACS biomaterials science & engineering · 2023Article
- Recent advances in 3D bioprinted cartilage-mimicking constructs for applications in tissue engineering.Materials today. Bio · 2023Review
- Flourishing tumor organoids: History, emerging technology, and application.Bioengineering & translational medicine · 2023Review
- Harnessing Biofabrication Strategies to Re-Surface Osteochondral Defects: Repair, Enhance, and Regenerate.Biomimetics (Basel, Switzerland) · 2023Review
- Systematic review on the application of 3D-bioprinting technology in orthoregeneration: current achievements and open challenges.Journal of experimental orthopaedics · 2022Review
- 3D bioprinting in tissue engineering and regenerative medicine.Cell and tissue banking · 2022Review
- The impact of microfluidics in high-throughput drug-screening applications.Biomicrofluidics · 2022Review
- Engineering Hydrogels for the Development of Three-Dimensional In Vitro Models.International journal of molecular sciences · 2022Review
- Recent advances in engineering hydrogels for niche biomimicking and hematopoietic stem cell culturing.Frontiers in bioengineering and biotechnology · 2022Review
- The promising rise of bioprinting in revolutionalizing medical science: Advances and possibilities.Regenerative therapy · 2021Review
- Review
- Narrative review of gene modification: applications in three-dimensional (3D) bioprinting.Annals of translational medicine · 2021Review
- Biofabrication Strategies for Musculoskeletal Disorders: Evolution towards Clinical Applications.Bioengineering (Basel, Switzerland) · 2021Review
- 3D bioprinting: novel approaches for engineering complex human tissue equivalents and drug testing.Essays in biochemistry · 2021Review
- 3D Bioprinting Strategies, Challenges, and Opportunities to Model the Lung Tissue Microenvironment and Its Function.Frontiers in bioengineering and biotechnology · 2021Review
- Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Over the past decade, bioprinting has emerged as a promising patterning strategy to organize cells and extracellular components both in two and three dimensions (2D and 3D) to engineer functional tissue mimicking constructs. So far, tissue printing has neither been used for 3D patterning of mesenchymal stem cells (MSCs) in multiphase growth factor embedded 3D hydrogels nor been investigated phenotypically in terms of simultaneous differentiation into different cell types within the same micropatterned 3D tissue constructs. Accordingly, we demonstrated a biochemical gradient by bioprinting nanoliter droplets encapsulating human MSCs, bone morphogenetic protein 2 (BMP-2), and transforming growth factor β1 (TGF- β1), engineering an anisotropic biomimetic fibrocartilage microenvironment. Assessment of the model tissue construct displayed multiphasic anisotropy of the incorporated biochemical factors after patterning. Quantitative real time polymerase chain reaction (qRT-PCR) results suggested genomic expression patterns leading to simultaneous differentiation of MSC populations into osteogenic and chondrogenic phenotype within the multiphasic construct, evidenced by upregulation of osteogenesis and condrogenesis related genes during in vitro culture. Comprehensive phenotypic network and pathway analysis results, which were based on genomic expression data, indicated activation of differentiation related mechanisms, via signaling pathways, including TGF, BMP, and vascular endothelial growth factor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.