ArticleJournal of the American Chemical Society2014
Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low viscosity fluids.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Harmonizing Peak Matching between Multidimensional NMR Spectra.Journal of chemical information and modeling · 2026Article
- Harmonizing Peak Matching Between Multidimensional NMR Spectra.bioRxiv : the preprint server for biology · 2026Article
- Advances in utilizing reverse micelles to investigate membrane proteins.Biochemical Society transactions · 2024Review
- Parahydrogen Polarization in Reverse Micelles and Application to Sensing of Protein-Ligand Binding.Journal of the American Chemical Society · 2024Article
- Characterization of 10MAG/LDAO reverse micelles: Understanding versatility for protein encapsulation.Biophysical chemistry · 2024Article
- Investigating protein-membrane interactions using native reverse micelles constructed from naturally sourced lipids.Protein science : a publication of the Protein Society · 2023Article
- Optimization of Biocompatibility for a Hydrophilic Biological Molecule Encapsulation System.Molecules (Basel, Switzerland) · 2022Article
- Article
- Extending the Detection Limit in Fragment Screening of Proteins Using Reverse Micelle Encapsulation.ACS chemical biology · 2019Article
- Site-Resolved and Quantitative Characterization of Very Weak Protein-Ligand Interactions.ACS chemical biology · 2019Article
- Characterizing Protein Hydration Dynamics Using Solution NMR Spectroscopy.Methods in enzymology · 2019Article
- Reverse Micelle Encapsulation of Proteins for NMR Spectroscopy.Methods in enzymology · 2019Article
- Accurate determination of rates from non-uniformly sampled relaxation data.Journal of biomolecular NMR · 2016Article
- Defining the Apoptotic Trigger: THE INTERACTION OF CYTOCHROME c AND CARDIOLIPIN.The Journal of biological chemistry · 2015Article
- Role of cavities and hydration in the pressure unfolding of T4 lysozyme.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- High-resolution NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Review
- Measurement and control of pH in the aqueous interior of reverse micelles.The journal of physical chemistry. B · 2014Article
- Reverse micelles as a platform for dynamic nuclear polarization in solution NMR of proteins.Journal of the American Chemical Society · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
An optimized reverse micelle surfactant system has been developed for solution nuclear magnetic resonance studies of encapsulated proteins and nucleic acids dissolved in low viscosity fluids. Comprising the nonionic 1-decanoyl-rac-glycerol and the zwitterionic lauryldimethylamine-N-oxide (10MAG/LDAO), this mixture is shown to efficiently encapsulate a diverse set of proteins and nucleic acids. Chemical shift analyses of these systems show that high structural fidelity is achieved upon encapsulation. The 10MAG/LDAO surfactant system reduces the molecular reorientation time for encapsulated macromolecules larger than ~20 kDa leading to improved overall NMR performance. The 10MAG/LDAO system can also be used for solution NMR studies of lipid-modified proteins. New and efficient strategies for optimization of encapsulation conditions are described. 10MAG/LDAO performs well in both the low viscosity pentane and ultralow viscosity liquid ethane and therefore will serve as a general surfactant system for initiating solution NMR studies of proteins and nucleic acids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.