ArticleRedox biology2014
Mechanism of faster NO scavenging by older stored red blood cells.
Article in Redox biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 52 citations in OpenAlex.
- Impact of Different Red Blood Cell Storage Solutions and Conditions on Cell Function and Viability: A Systematic Review.Biomolecules · 2024Pooled it
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- Nitric oxide administration during paediatric cardiopulmonary bypass: a randomised controlled trial.Intensive care medicine · 2016Trial
- SIRPα extracellular domain shed from neutrophils by ADAM10 aggravates sepsis via initiating nitric oxide release from erythrocyte.Journal of advanced research · 2026Article
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- Perioperative red blood cell infusion and deep vein thrombosis in patients with femoral and pelvic fractures: a propensity score matching.Journal of orthopaedic surgery and research · 2021Article
- Innate coagulability changes with age in stored packed red blood cells.Thrombosis research · 2020Article
- Red blood cells: the forgotten player in hemostasis and thrombosis.Journal of thrombosis and haemostasis : JTH · 2019Review
- Red blood cell storage lesion: causes and potential clinical consequences.Blood transfusion = Trasfusione del sangue · 2019Review
- Is It Possible to Reverse the Storage-Induced Lesion of Red Blood Cells?Frontiers in physiology · 2018Article
- Duration of red blood cell storage and inflammatory marker generation.Blood transfusion = Trasfusione del sangue · 2017Review
- Enhancing uniformity and overall quality of red cell concentrate with anaerobic storage.Blood transfusion = Trasfusione del sangue · 2017Article
- Role of red blood cells in haemostasis and thrombosis.ISBT science series · 2017Article
- Transfusion as an Inflammation Hit: Knowns and Unknowns.Frontiers in immunology · 2016Review
- In a canine pneumonia model of exchange transfusion, altering the age but not the volume of older red blood cells markedly alters outcome.Transfusion · 2015Article
- Checkpoint kinase 1 inhibitors as targeted molecular agents for clear cell carcinoma of the ovary.Oncology letters · 2015Article
- Red blood cell washing, nitrite therapy, and antiheme therapies prevent stored red blood cell toxicity after trauma-hemorrhage.Free radical biology & medicine · 2015Article
- Identification of signalling cascades involved in red blood cell shrinkage and vesiculation.Bioscience reports · 2015Article
- Established and theoretical factors to consider in assessing the red cell storage lesion.Blood · 2015Article
- The Association Between Mean Corpuscular Hemoglobin Concentration and Prognosis in Patients with Acute Pulmonary Embolism: A Retrospective Cohort Study.Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/HemostasisArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
unlabelledThe blood storage lesion involves morphological and biochemical changes of red blood cells (RBCs) that occur during storage. These include conversion of the biconcave disc morphology to a spherical one, decreased mean corpuscular hemoglobin concentration, varied mean corpuscular volume, reduced integrity of the erythrocyte membrane with formation of microparticles, and increased cell-free hemoglobin. We studied the extent that older stored red blood cells scavenge nitric oxide (NO) faster than fresher stored red blood cells. Using electron paramagnetic resonance spectroscopy and stopped-flow absorption spectroscopy to measure the rate of NO uptake and reaction with hemoglobin in red cells, we found that older stored red blood cells scavenge NO about 1.8 times faster than fresher ones. Based on these experimental data, we simulated NO scavenging by fresher or older stored red blood cells with a biconcave or spherical geometry, respectively, in order to explore the mechanism of NO scavenging related to changes that occur during blood storage. We found that red blood cells with a spherical geometry scavenges NO about 2 times slower than ones with a biconcave geometry, and a smaller RBC hemoglobin concentration or volume increases NO scavenging by red blood cells. Our simulations demonstrate that even the most extreme possible changes in mean corpuscular hemoglobin concentration and mean corpuscular volume that favor increased NO scavenging are insufficient to account for what is observed experimentally. Therefore, RBC membrane permeability must increase during storage and we find that the permeability is likely to increase between 5 and 70 fold. Simulations using a two-dimensional blood vessel show that even a 5-fold increase in membrane permeability to NO can reduce NO bioavailability at the smooth muscle.
backgroundTransfusion of older stored blood may be harmful.
resultsOlder stored red blood cells scavenge nitric oxide more than fresher cells.
conclusionAs stored red blood cells age, structural and biochemical changes occur that lead to faster scavenging. SIGNIFICANCE: Increased nitric oxide scavenging by red blood cells as a function of storage age contributes to deleterious effects upon transfusion.
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Registered trials
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