ArticleMicrobiology (Reading, England)2014
The protein tyrosine kinases EpsB and PtkA differentially affect biofilm formation in Bacillus subtilis.
Article in Microbiology (Reading, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Evidence that glycopolymer transferases promote peptidoglycan hydrolysis inbioRxiv : the preprint server for biology · 2025Article
- Molecular profiling and bioinformatics approaches of biofilm formation in ionizing radiation-resistant Bacillus subtilis, isolated from geothermal spring in Ramsar, the North of Iran.World journal of microbiology & biotechnology · 2025Article
- Coenzyme A biosynthesis inmBio · 2024Article
- Chance Favors the Prepared Genomes: Horizontal Transfer Shapes the Emergence of Antibiotic Resistance Mutations in Core Genes.Molecular biology and evolution · 2023Article
- Giving a signal: how protein phosphorylation helps Bacillus navigate through different life stages.FEMS microbiology reviews · 2023Review
- Review
- Bacillus subtilis biofilm formation and social interactions.Nature reviews. Microbiology · 2021Review
- Effects of an EPS Biosynthesis Gene Cluster ofMicroorganisms · 2021Article
- The exopolysaccharide-eDNA interaction modulates 3D architecture of Bacillus subtilis biofilm.BMC microbiology · 2020Article
- Structural Analysis of the Hanks-Type Protein Kinase YabT FromFrontiers in microbiology · 2018Article
- General and Modular Strategy for Designing Potent, Selective, and Pharmacologically Compliant Inhibitors of Rhomboid Proteases.Cell chemical biology · 2017Article
- Increasing the bioflocculant production and identifying the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis.Microbial cell factories · 2017Article
- Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus.Marine drugs · 2017Article
- Low Concentrations of Vitamin C Reduce the Synthesis of Extracellular Polymers and Destabilize Bacterial Biofilms.Frontiers in microbiology · 2017Article
- Substrate Specificity of theFrontiers in microbiology · 2016Article
- Spatio-temporal assembly of functional mineral scaffolds within microbial biofilms.NPJ biofilms and microbiomes · 2016Article
- Second Messenger Signaling in Bacillus subtilis: Accumulation of Cyclic di-AMP Inhibits Biofilm Formation.Frontiers in microbiology · 2016Article
- Tyrosine 601 of Bacillus subtilis DnaK Undergoes Phosphorylation and Is Crucial for Chaperone Activity and Heat Shock Survival.Frontiers in microbiology · 2016Article
- The Bacterial Tyrosine Kinase Activator TkmA Contributes to Biofilm Formation Largely Independently of the Cognate Kinase PtkA in Bacillus subtilis.Journal of bacteriology · 2015Article
- Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The Gram-positive soil bacterium Bacillus subtilis is able to choose between motile and sessile lifestyles. The sessile way of life, also referred to as biofilm, depends on the formation of an extracellular polysaccharide matrix and some extracellular proteins. Moreover, a significant proportion of cells in a biofilm form spores. The first two genes of the 15-gene operon for extracellular polysaccharide synthesis, epsA and epsB, encode a putative transmembrane modulator protein and a putative protein tyrosine kinase, respectively, with similarity to the TkmA/PtkA modulator/kinase couple. Here we show that the putative kinase EpsB is required for the formation of structured biofilms. However, an epsB mutant is still able to form biofilms. As shown previously, a ptkA mutant is also partially defective in biofilm formation, but this defect is related to spore formation in the biofilm. The absence of both kinases resulted in a complete loss of biofilm formation. Thus, EpsB and PtkA fulfil complementary functions in biofilm formation. The activity of bacterial protein tyrosine kinases depends on their interaction with modulator proteins. Our results demonstrate the specific interaction between the putative kinase EpsB and its modulator protein EpsA and suggest that EpsB activity is stimulated by its modulator EpsA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.