ArticleAntimicrobial agents and chemotherapy2014
Phenethyl isothiocyanate inhibits shiga toxin production in enterohemorrhagic Escherichia coli by stringent response induction.
Article in Antimicrobial agents and chemotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- L-arabinose: a strategic modulator of pathogen virulence for food system safety.Archives of microbiology · 2026Review
- Phytochemicals Controlling EnterohemorrhagicInternational journal of molecular sciences · 2025Review
- Targeting the bacterial stringent response to combat human pathogens.Frontiers in immunology · 2025Review
- How to Tackle Bacteriophages: The Review of Approaches with Mechanistic Insight.International journal of molecular sciences · 2023Review
- Increased Levels of (p)ppGpp Correlate with Virulence and Biofilm Formation, but Not with Growth, in Strains of UropathogenicInternational journal of molecular sciences · 2023Article
- Signals triggering prophage induction in the gut microbiota.Molecular microbiology · 2022Review
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- Dietary Isothiocyanates, Sulforaphane and 2-Phenethyl Isothiocyanate, Effectively ImpairInternational journal of molecular sciences · 2021Article
- Prophage Activation in the Intestine: Insights Into Functions and Possible Applications.Frontiers in microbiology · 2021Review
- Vitamin K Analogs Influence the Growth and Virulence Potential of Enterohemorrhagic Escherichia coli.Applied and environmental microbiology · 2020Article
- Effect ofNutrients · 2020Article
- Induction of the Stringent Response Underlies the Antimicrobial Action of Aliphatic Isothiocyanates.Frontiers in microbiology · 2020Article
- Various modes of action of dietary phytochemicals, sulforaphane and phenethyl isothiocyanate, on pathogenic bacteria.Scientific reports · 2019Article
- Relevance of Bacteriophage 933W in the Development of Hemolytic Uremic Syndrome (HUS).Frontiers in microbiology · 2018Article
- Cinnamon Oil Inhibits Shiga Toxin Type 2 Phage Induction and Shiga Toxin Type 2 Production in Escherichia coli O157:H7.Applied and environmental microbiology · 2016Article
- Isothiocyanates as effective agents against enterohemorrhagic Escherichia coli: insight to the mode of action.Scientific reports · 2016Article
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Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pathogenicity of enterohemorrhagic Escherichia coli (EHEC) depends on production of Shiga toxins, which are encoded by stx genes located in the genomes of lambdoid prophages. Efficient expression of these genes requires prophage induction and lytic development of phages. Treatment of EHEC infections is problematic due to not only the resistance of various strains to antibiotics but also the fact that many antibiotics cause prophage induction, thus resulting in high-level expression of stx genes. Here we report that E. coli growth, Shiga toxin-converting phage development, and production of the toxin by EHEC are strongly inhibited by phenethyl isothiocyanate (PEITC). We demonstrate that PEITC induces the stringent response in E. coli that is mediated by massive production of a global regulator, guanosine tetraphosphate (ppGpp). The stringent response induction arises most probably from interactions of PEITC with amino acids and from amino acid deprivation-mediated activation of ppGpp synthesis. In mutants unable to synthesize ppGpp, development of Shiga toxin-converting phages and production of Shiga toxin are significantly enhanced. Therefore, ppGpp, which appears at high levels in bacterial cells after stimulation of its production by PEITC, is a negative regulator of EHEC virulence and at the same time efficiently inhibits bacterial growth. This is in contrast to stimulation of virulence of different bacteria by this nucleotide reported previously by others.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.