ArticlePlant physiology2014
Vegetative and sperm cell-specific aquaporins of Arabidopsis highlight the vacuolar equipment of pollen and contribute to plant reproduction.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 65 citations in OpenAlex.
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Genetic control of generative cell shape by DUO1 in Arabidopsis.Plant reproduction · 2023Article
- DMP8 and 9 regulate HAP2/GCS1 trafficking for the timely acquisition of sperm fusion competence.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- TheInternational journal of molecular sciences · 2021Article
- Article
- Versatile Roles of Aquaporins in Plant Growth and Development.International journal of molecular sciences · 2020Review
- Fertilization in flowering plants: an odyssey of sperm cell delivery.Plant molecular biology · 2020Review
- The ER-localized aquaporin SIP2;1 is involved in pollen germination and pollen tube elongation in Arabidopsis thaliana.Plant molecular biology · 2019Article
- ArabidopsisPlant physiology · 2018Article
- The ADAPTOR PROTEIN-3 Complex Mediates Pollen Tube Growth by Coordinating Vacuolar Targeting and Organization.Plant physiology · 2018Article
- Plant and Mammal Aquaporins: Same but Different.International journal of molecular sciences · 2018Review
- Genome-wide identification, characterization, and expression profile of aquaporin gene family in flax (Linum usitatissimum).Scientific reports · 2017Article
- Understanding Aquaporin Transport System in Eelgrass (Frontiers in plant science · 2017Article
- Signaling with Ions: The Keystone for Apical Cell Growth and Morphogenesis in Pollen Tubes.Plant physiology · 2017Review
- Pollen aquaporins: What are they there for?Plant signaling & behavior · 2016Article
- Importance of organellar proteins, protein translocation and vesicle transport routes for pollen development and function.Plant reproduction · 2016Review
- The C-terminal motif of SiAGO1b is required for the regulation of growth, development and stress responses in foxtail millet (Setaria italica (L.) P. Beauv).Journal of experimental botany · 2016Article
- Pollen-Specific Aquaporins NIP4;1 and NIP4;2 Are Required for Pollen Development and Pollination in Arabidopsis thaliana.The Plant cell · 2016Article
- Tonoplast Aquaporins Facilitate Lateral Root Emergence.Plant physiology · 2016Article
- The Roles of Aquaporins in Plant Stress Responses.Journal of developmental biology · 2016Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The water and nutrient status of pollen is crucial to plant reproduction. Pollen grains of Arabidopsis (Arabidopsis thaliana) contain a large vegetative cell and two smaller sperm cells. Pollen grains express AtTIP1;3 and AtTIP5;1, two members of the Tonoplast Intrinsic Protein subfamily of aquaporins. To address the spatial and temporal expression pattern of the two homologs, C-terminal fusions of AtTIP1;3 and AtTIP5;1 with green fluorescent protein and mCherry, respectively, were expressed in transgenic Arabidopsis under the control of their native promoter. Confocal laser scanning microscopy revealed that AtTIP1;3 and AtTIP5;1 are specific for the vacuoles of the vegetative and sperm cells, respectively. The tonoplast localization of AtTIP5;1 was established by reference to fluorescent protein markers for the mitochondria and vacuoles of sperm and vegetative cells and is at variance with the claim that AtTIP5;1 is localized in vegetative cell mitochondria. AtTIP1;3-green fluorescent protein and AtTIP5;1-mCherry showed concomitant expression, from first pollen mitosis up to pollen tube penetration in the ovule, thereby revealing the dynamics of vacuole morphology in maturating and germinating pollen. Transfer DNA insertion mutants for either AtTIP1;3 or AtTIP5;1 showed no apparent growth phenotype and had no significant defect in male transmission of the mutated alleles. By contrast, a double knockout displayed an abnormal rate of barren siliques, this phenotype being more pronounced under limited water or nutrient supply. The overall data indicate that vacuoles of vegetative and sperm cells functionally interact and contribute to male fertility in adverse environmental conditions.
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