ArticleInfection and immunity2014
The δ subunit of RNA polymerase guides promoter selectivity and virulence in Staphylococcus aureus.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 38 citations in OpenAlex.
- Profiling of genetic determinants required for fitness of community-associated methicillin-resistantMicrobiology spectrum · 2026Article
- RpoE mediates environmental stress tolerance and biofilm formation in foodborneCurrent research in food science · 2026Article
- Structural basis of promoter recognition by Staphylococcus aureus RNA polymerase.Nature communications · 2024Article
- Toolbox of Characterized Genetic Parts forACS synthetic biology · 2024Article
- The identification of two M20B family peptidases required for full virulence inFrontiers in cellular and infection microbiology · 2023Article
- Loss ofMicroorganisms · 2022Article
- An Ex Vivo Model for Assessing Growth and Survivability of Staphylococcus aureus in Whole Human Blood.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Isopropoxy Benzene Guanidine KillsFrontiers in microbiology · 2021Article
- A FAS solution to a DEAD case.PLoS genetics · 2020Article
- The DEAD-box RNA helicase CshA is required for fatty acid homeostasis in Staphylococcus aureus.PLoS genetics · 2020Article
- The dormancy-specific regulator, SutA, is intrinsically disordered and modulates transcription initiation in Pseudomonas aeruginosa.Molecular microbiology · 2019Article
- Identification of a unique transcriptional architecture for the sigS operon in Staphylococcus aureus.FEMS microbiology letters · 2018Article
- Solution structure of domain 1.1 of the σThe Journal of biological chemistry · 2017Article
- The ω Subunit Governs RNA Polymerase Stability and Transcriptional Specificity in Staphylococcus aureus.Journal of bacteriology · 2017Article
- A Novel Function of δ Factor from Bacillus subtilis as a Transcriptional Repressor.The Journal of biological chemistry · 2016Article
- TSS-EMOTE, a refined protocol for a more complete and less biased global mapping of transcription start sites in bacterial pathogens.BMC genomics · 2016Article
- Regulatory Requirements for Staphylococcus aureus Nitric Oxide Resistance.Journal of bacteriology · 2016Article
- Parallel Evolution in Streptococcus pneumoniae Biofilms.Genome biology and evolution · 2016Article
- Towards the complete small RNome ofMicrobial genomics · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In Gram-positive bacteria, and particularly the Firmicutes, the DNA-dependent RNA polymerase (RNAP) complex contains an additional subunit, termed the δ factor, or RpoE. This enigmatic protein has been studied for more than 30 years for various organisms, but its function is still not well understood. In this study, we investigated its role in the major human pathogen Staphylococcus aureus. We showed conservation of important structural regions of RpoE in S. aureus and other species and demonstrated binding to core RNAP that is mediated by the β and/or β' subunits. To identify the impact of the δ subunit on transcription, we performed transcriptome sequencing (RNA-seq) analysis and observed 191 differentially expressed genes in the rpoE mutant. Ontological analysis revealed, quite strikingly, that many of the downregulated genes were known virulence factors, while several mobile genetic elements (SaPI5 and prophage SA3usa) were strongly upregulated. Phenotypically, the rpoE mutant had decreased accumulation and/or activity of a number of key virulence factors, including alpha toxin, secreted proteases, and Panton-Valentine leukocidin (PVL). We further observed significantly decreased survival of the mutant in whole human blood, increased phagocytosis by human leukocytes, and impaired virulence in a murine model of infection. Collectively, our results demonstrate that the δ subunit of RNAP is a critical component of the S. aureus transcription machinery and plays an important role during infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.