ArticleRSC advances2014
Quantitative Fluorescence Assays Using a Self-Powered Paper-Based Microfluidic Device and a Camera-Equipped Cellular Phone.
Article in RSC advances, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Paper and thread as media for the frugal detection of urinary tract infections (UTIs).Analytical and bioanalytical chemistry · 2022Review
- Recent trends in smartphone-based detection for biomedical applications: a review.Analytical and bioanalytical chemistry · 2021Review
- Increasing the packing density of assays in paper-based microfluidic devices.Biomicrofluidics · 2021Review
- Emerging trends in paper spray mass spectrometry: Microsampling, storage, direct analysis, and applications.Mass spectrometry reviews · 2020Review
- A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for Salmonella DNA detection.RSC advances · 2018Article
- Challenges in paper-based fluorogenic optical sensing with smartphones.Nano convergence · 2018Review
- Flexible and disposable paper- and plastic-based gel micropads for nematode handling, imaging, and chemical testing.APL bioengineering · 2017Article
- Plasmonics Enhanced Smartphone Fluorescence Microscopy.Scientific reports · 2017Article
- A low-cost smartphone-based platform for highly sensitive point-of-care testing with persistent luminescent phosphors.Lab on a chip · 2017Article
- A Review on Microfluidic Paper-Based Analytical Devices for Glucose Detection.Sensors (Basel, Switzerland) · 2016Review
- A chemiluminescent platform for smartphone monitoring of HMethods (San Diego, Calif.) · 2016Article
- Ultra-portable, wireless smartphone spectrometer for rapid, non-destructive testing of fruit ripeness.Scientific reports · 2016Article
- Paper-based analytical devices for environmental analysis.The Analyst · 2016Review
- Design, Synthesis, and Characterization of Small-Molecule Reagents That Cooperatively Provide Dual Readouts for Triaging and, When Necessary, Quantifying Point-of-Need Enzyme Assays.The Journal of organic chemistry · 2015Article
- A Strategy for Minimizing Background Signal in Autoinductive Signal Amplification Reactions for Point-of-Need Assays.Analytical methods : advancing methods and applications · 2015Article
- Quantitative detection of pharmaceuticals using a combination of paper microfluidics and wavelength modulated Raman spectroscopy.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Fluorescence assays often require specialized equipment and, therefore, are not easily implemented in resource-limited environments. Herein we describe a point-of-care assay strategy in which fluorescence in the visible region is used as a readout, while a camera-equipped cellular phone is used to capture the fluorescent response and quantify the assay. The fluorescence assay is made possible using a paper-based microfluidic device that contains an internal fluidic battery, a surface-mount LED, a 2-mm section of a clear straw as a cuvette, and an appropriately-designed small molecule reagent that transforms from weakly fluorescent to highly fluorescent when exposed to a specific enzyme biomarker. The resulting visible fluorescence is digitized by photographing the assay region using a camera-equipped cellular phone. The digital images are then quantified using image processing software to provide sensitive as well as quantitative results. In a model 30 min assay, the enzyme β-D-galactosidase was measured quantitatively down to 700 pM levels. This Communication describes the design of these types of assays in paper-based microfluidic devices and characterizes the key parameters that affect the sensitivity and reproducibility of the technique.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.