ArticlePloS one2014
Activity-dependent adenosine release may be linked to activation of Na(+)-K(+) ATPase: an in vitro rat study.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Local versus global sleep organization and the quest to determine sleep function.Neurobiology of sleep and circadian rhythms · 2025Article
- Extracellular ATP/adenosine dynamics in the brain and its role in health and disease.Frontiers in cell and developmental biology · 2023Review
- Ketamine decreases neuronally released glutamate via retrograde stimulation of presynaptic adenosine A1 receptors.Molecular psychiatry · 2021Article
- ATP and adenosine-Two players in the control of seizures and epilepsy development.Progress in neurobiology · 2021Review
- The Purine Salvage Pathway and the Restoration of Cerebral ATP: Implications for Brain Slice Physiology and Brain Injury.Neurochemical research · 2019Review
- Detection of phasic dopamine by D1 and D2 striatal medium spiny neurons.The Journal of physiology · 2017Article
- Blood purine measurements as a rapid real-time indicator of reversible brain ischaemia.Purinergic signalling · 2017Observational
- Transient Adenosine Release Is Modulated by NMDA and GABAACS chemical neuroscience · 2017Article
- Reducing Extracellular CaFrontiers in neural circuits · 2017Article
- Adenosine APurinergic signalling · 2016Article
- Sodium pump organization in dendritic spines.Neurophotonics · 2016Article
- Adenosine A2B receptor activation stimulates glucose uptake in the mouse forebrain.Purinergic signalling · 2015Article
- Sensing Positive versus Negative Reward Signals through Adenylyl Cyclase-Coupled GPCRs in Direct and Indirect Pathway Striatal Medium Spiny Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Acute hyperammonemia and systemic inflammation is associated with increased extracellular brain adenosine in rats: a biosensor study.Neurochemical research · 2015Article
- Strategies of Functional Foods Promote Sleep in Human Being.Current signal transduction therapy · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In the brain, extracellular adenosine increases as a result of neuronal activity. The mechanisms by which this occurs are only incompletely understood. Here we investigate the hypothesis that the Na(+) influxes associated with neuronal signalling activate the Na(+)-K(+) ATPase which, by consuming ATP, generates intracellular adenosine that is then released via transporters. By measuring adenosine release directly with microelectrode biosensors, we have demonstrated that AMPA-receptor evoked adenosine release in basal forebrain and cortex depends on extracellular Na(+). We have simultaneously imaged intracellular Na(+) and measured adenosine release. The accumulation of intracellular Na(+) during AMPA receptor activation preceded adenosine release by some 90 s. By removing extracellular Ca(2+), and thus preventing indiscriminate neuronal activation, we used ouabain to test the role of the Na(+)-K(+) ATPase in the release of adenosine. Under conditions which caused a Na(+) influx, brief applications of ouabain increased the accumulation of intracellular Na(+) but conversely rapidly reduced extracellular adenosine levels. In addition, ouabain greatly reduced the amount of adenosine released during application of AMPA. Our data therefore suggest that activity of the Na(+)-K(+) ATPase is directly linked to the efflux of adenosine and could provide a universal mechanism that couples adenosine release to neuronal activity. The Na(+)-K(+) ATPase-dependent adenosine efflux is likely to provide adenosine-mediated activity-dependent negative feedback that will be important in many diverse functional contexts including the regulation of sleep.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.