ArticlePloS one2014
Mapping organelle motion reveals a vesicular conveyor belt spatially replenishing secretory vesicles in stimulated chromaffin cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Decoding the role of microtubules: a trafficking road for vesicle.Theranostics · 2025Review
- Live-Cell Superresolution Imaging of Retrograde Axonal Trafficking Using Pulse-Chase Labeling in Cultured Hippocampal Neurons.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Spatial redistribution of neurosecretory vesicles upon stimulation accelerates their directed transport to the plasma membrane.PloS one · 2022Article
- Motility Plays an Important Role in the Lifetime of Mammalian Lipid Droplets.International journal of molecular sciences · 2021Review
- Quasi-steady-state reduction of a model for cytoplasmic transport of secretory vesicles in stimulated chromaffin cells.Journal of mathematical biology · 2021Article
- Multiple Mechanisms Driving F-actin-Dependent Transport of Organelles to and From Secretory Sites in Bovine Chromaffin Cells.Frontiers in cellular neuroscience · 2018Article
- The role of F-actin in the transport and secretion of chromaffin granules: an historic perspective.Pflugers Archiv : European journal of physiology · 2018Review
- Presynaptic morphology and vesicular composition determine vesicle dynamics in mouse central synapses.eLife · 2017Article
- Kinetics of milk lipid droplet transport, growth, and secretion revealed by intravital imaging: lipid droplet release is intermittently stimulated by oxytocin.Molecular biology of the cell · 2017Article
- The Munc18-1 domain 3a hinge-loop controls syntaxin-1A nanodomain assembly and engagement with the SNARE complex during secretory vesicle priming.The Journal of cell biology · 2016Article
- Control of autophagosome axonal retrograde flux by presynaptic activity unveiled using botulinum neurotoxin type a.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Vesicle Motion during Sustained Exocytosis in Chromaffin Cells: Numerical Model Based on Amperometric Measurements.PloS one · 2015Article
- Lipid droplet motility and organelle contacts.Contact (Thousand Oaks (Ventura County, Calif.))Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How neurosecretory cells spatially adjust their secretory vesicle pools to replenish those that have fused and released their hormonal content is currently unknown. Here we designed a novel set of image analyses to map the probability of tracked organelles undergoing a specific type of movement (free, caged or directed). We then applied our analysis to time-lapse z-stack confocal imaging of secretory vesicles from bovine Chromaffin cells to map the global changes in vesicle motion and directionality occurring upon secretagogue stimulation. We report a defined region abutting the cortical actin network that actively transports secretory vesicles and is dissipated by actin and microtubule depolymerizing drugs. The directionality of this "conveyor belt" towards the cell surface is activated by stimulation. Actin and microtubule networks therefore cooperatively probe the microenvironment to transport secretory vesicles to the periphery, providing a mechanism whereby cells globally adjust their vesicle pools in response to secretagogue stimulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.