ArticlePloS one2014
Sex and genetic factors determine osteoblastic differentiation potential of murine bone marrow stromal cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- Pooled it
- Engineering bioinspired, high-density collagen microgels with tunable intrafibrillar mineralization for accelerated osteogenesis in vitro and bone regeneration in vivo.Acta biomaterialia · 2026Article
- Inactivation of Hes1 in Skeletal Undifferentiated Cells Increases Bone Volume.Endocrinology · 2026Article
- An in vivo assay for osteoclast activity using mouse calvaria.Animal models and experimental medicine · 2026Article
- COX-2 inhibition as a therapeutic strategy for bone loss in Staphylococcus aureus osteomyelitis.Molecular medicine (Cambridge, Mass.) · 2025Article
- Sex-dependent variation in bone adaptation: from degeneration to regeneration.Journal of orthopaedic translation · 2025Review
- Lysine specific demethylase 1 conditional myeloid cell knockout mice have decreased osteoclast differentiation due to increased IFN-JBMR plus · 2025Article
- Isolation and Culture of Osteoblasts.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Evaluation of Potential Roles of Zinc Finger Homeobox 3 (Zfhx3) Expressed in Chondrocytes and Osteoblasts on Skeletal Growth in Mice.Calcified tissue international · 2024Article
- Sexual Dimorphism in the Musculoskeletal System: Sex Hormones and Beyond.Journal of the Endocrine Society · 2024Review
- The potency of mesenchymal stem/stromal cells: does donor sex matter?Stem cell research & therapy · 2024Review
- Estrogen Deficiency Exacerbates Traumatic Heterotopic Ossification in Mice.Journal of inflammation research · 2024Article
- Article
- PDGF inhibits BMP2-induced bone healing.NPJ Regenerative medicine · 2023Article
- Sexing Bones: Improving Transparency of Sex Reporting to Address Bias Within Preclinical Studies.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- Activation of canonical Wnt signaling accelerates intramembranous bone regeneration in male mice.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- New insights into the role of matrix metalloproteinase 3 (MMP3) in bone.FASEB bioAdvances · 2022Article
- Universal or Personalized Mesenchymal Stem Cell Therapies: Impact of Age, Sex, and Biological Source.Cells · 2022Review
- Age-Progressive and Gender-Dependent Bone Phenotype in Mice Lacking Both Ebf1 and Ebf2 in Prrx1-Expressing Mesenchymal Cells.Calcified tissue international · 2022Article
- Evaluation of the Age- and Sex-Related Changes of the Osteogenic Differentiation Potentials of Healthy Bone Marrow-Derived Mesenchymal Stem Cells.Medicina (Kaunas, Lithuania) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Sex and genetic factors determine skeletal mass, and we tested whether bone histomorphometric parameters were sexually dimorphic in femurs from 1 to 6 month old C57BL/6 mice. Trabecular bone volume declined more rapidly in female mice than in male littermates because of enhanced bone resorption. Although bone formation was not different between sexes, female mice exhibited a higher number of osteoblasts than male littermates, suggesting that osteoblasts from female mice may have a reduced ability to form bone. To determine the impact of sex on osteoblastogenesis, we investigated the potential for osteoblastic differentiation of bone marrow stromal cells from C57BL/6, Friend leukemia virus-B (FVB), C3H/HeJ and BALB/c mice of both sexes. Bone marrow stromal cells from female FVB, C57BL/6 and C3H/HeJ mice exhibited lower Alpl and Osteocalcin expression and alkaline phosphatase activity, and formed fewer mineralized nodules than cells from male littermates. Proliferative capacity was greater in cells from male than female C57BL/6, but not FVB, mice. Sorting of bone marrow stromal cells from mice expressing an α-Smooth muscle actin-green fluorescent protein transgene, revealed a higher yield of mesenchymal stem cells in cultures from male mice than in those from female littermates. Sex had a modest impact on osteoblastic differentiation of mesenchymal stem cells. To determine the influence of sex and genetic factors on osteoblast function, calvarial osteoblasts were harvested from C57BL/6, FVB, C3H/HeJ and BALB/c mice. Alpl expression and activity were lower in osteoblasts from C57BL/6 and C3H/HeJ, but not FVB or BALB/c, female mice than in cells from littermates. Sex had no effect on osteoclastogenesis of bone marrow cultures of C57BL/6 mice, but osteoblasts from female mice exhibited higher Rankl and lower Opg expression than cells from male littermates. In conclusion, osteoblastogenesis is sexually dimorphic and influenced by genetic factors.
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Registered trials
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