ArticlePloS one2014
Probing oral microbial functionality--expression of spxB in plaque samples.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 22 citations in OpenAlex.
- HApplied and environmental microbiology · 2026Article
- Identification and Characterization of Negative Regulators of Rgg1518 Quorum Sensing in Streptococcus pneumoniae.Journal of bacteriology · 2023Article
- Simultaneous Chemical Mapping of Live Biofilm Microenvironmental pH and Hydrogen Peroxide in Real Time with a Triple Scanning Electrochemical Microscopy Tip.Analytical chemistry · 2023Article
- Probiotics During the Therapeutic Management of Periodontitis.Advances in experimental medicine and biology · 2022Review
- Post-translational modification of Streptococcus sanguinis SpxB influences protein solubility and HMolecular oral microbiology · 2021Article
- Quantification and gene expression ofThe Saudi dental journal · 2021Article
- Induction of Apoptotic Cell Death by Oral Streptococci in Human Periodontal Ligament Cells.Frontiers in microbiology · 2021Article
- Where in the world do bacteria experience oxidative stress?Environmental microbiology · 2019Review
- Live and let die: Hydrogen peroxide production by the commensal flora and its role in maintaining a symbiotic microbiome.Molecular oral microbiology · 2018Review
- Distinct Regulatory Role of Carbon Catabolite Protein A (CcpA) in Oral StreptococcalJournal of bacteriology · 2018Article
- Plasticity of the Pyruvate Node Modulates Hydrogen Peroxide Production and Acid Tolerance in Multiple Oral Streptococci.Applied and environmental microbiology · 2018Article
- The activation of the oxidative stress response transcription factor SKN-1 in Caenorhabditis elegans by mitis group streptococci.PloS one · 2018Article
- Real-Time Metabolic Interactions between Two Bacterial Species Using a Carbon-Based pH Microsensor as a Scanning Electrochemical Microscopy Probe.Analytical chemistry · 2017Article
- The road less traveled - defining molecular commensalism with Streptococcus sanguinis.Molecular oral microbiology · 2017Review
- [The origin of hydrogen peroxide in oral cavity and its role in oral microecology balance].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2017Article
- Streptococcus sanguinis induces neutrophil cell death by production of hydrogen peroxide.PloS one · 2017Article
- Two-component system VicRK regulates functions associated with establishment of Streptococcus sanguinis in biofilms.Infection and immunity · 2014Article
- Comparison of genes required for H2O2 resistance in Streptococcus gordonii and Streptococcus sanguinis.Microbiology (Reading, England) · 2014Article
- The impact of horizontal gene transfer on the adaptive ability of the human oral microbiome.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The Human Oral Microbiome Database (HOMD) provides an extensive collection of genome sequences from oral bacteria. The sequence information is a static snapshot of the microbial potential of the so far sequenced species. A major challenge is to connect the microbial potential encoded in the metagenome to an actual function in the in vivo oral biofilm. In the present study we took a reductionist approach and identified a considerably conserved metabolic gene, spxB to be encoded by a majority of oral streptococci using the HOMD metagenome information. spxB encodes the pyruvate oxidase responsible for the production of growth inhibiting amounts of hydrogen peroxide (H2O2) and has previously been shown as important in the interspecies competition in the oral biofilm. Here we demonstrate a strong correlation of H2O2 production and the presence of the spxB gene in dental plaque. Using Real-Time RT PCR we show that spxB is expressed in freshly isolated human plaque samples from several donors and that the expression is relative constant when followed over time in one individual. This is the first demonstration of an oral community encoded gene expressed in vivo suggesting a functional role of spxB in oral biofilm physiology. This also demonstrates a possible strategy to connect the microbial potential of the metagenome to its functionality in future studies by identifying similar highly conserved genes in the oral microbial community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.