ArticlePloS one2014
Prdm8 regulates the morphological transition at multipolar phase during neocortical development.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- DNA Methylation in Newborns and Maternal Smoking in Pregnancy: Genome-wide Consortium Meta-analysis.American journal of human genetics · 2016Pooled it
- Assessment of the Association of Periodontitis and Diabetes Mellitus with Alzheimer's Disease in a Mouse Model.International journal of molecular sciences · 2026Article
- The Roles of the PRDM Family in the Neuro-Motor System.Biomolecules · 2026Review
- Transcriptomic profiles of susceptibility and resilience to stress in the amygdala and hippocampus of male rats.Neurobiology of stress · 2025Article
- Prenatal nicotine exposure during pregnancy results in adverse neurodevelopmental alterations and neurobehavioral deficits.Advances in drug and alcohol research · 2023Review
- The second family affected with a PRDM8-related disease.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2022Article
- The genome-wide impact of trisomy 21 on DNA methylation and its implications for hematopoiesis.Nature communications · 2021Article
- Loss of BAF Complex in Developing Cortex Perturbs Radial Neuronal Migration in a WNT Signaling-Dependent Manner.Frontiers in molecular neuroscience · 2021Article
- Review
- Sliced Human Cortical Organoids for Modeling Distinct Cortical Layer Formation.Cell stem cell · 2020Article
- Involvement of Netrins and Their Receptors in Neuronal Migration in the Cerebral Cortex.Frontiers in cell and developmental biology · 2020Review
- Review
- Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells.Scientific reports · 2018Article
- Genetic and Molecular Approaches to Study Neuronal Migration in the Developing Cerebral Cortex.Brain sciences · 2017Review
- Neuronal Polarity in the Embryonic Mammalian Cerebral Cortex.Frontiers in cellular neuroscience · 2017Review
- Decoding the molecular mechanisms of neuronal migration using in utero electroporation.Medical molecular morphology · 2016Review
- Knockout mouse production assisted by Blm knockdown.The Journal of reproduction and development · 2016Article
- An emerging role for prdm family genes in dorsoventral patterning of the vertebrate nervous system.Neural development · 2015Review
- Spatiotemporal expression of Prdm genes during Xenopus development.Cytotechnology · 2015Article
- Molecular Pathways Underlying Projection Neuron Production and Migration during Cerebral Cortical Development.Frontiers in neuroscience · 2015Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we found that the PR domain protein Prdm8 serves as a key regulator of the length of the multipolar phase by controlling the timing of morphological transition. We used a mouse line with expression of Prdm8-mVenus reporter and found that Prdm8 is predominantly expressed in the middle and upper intermediate zone during both the late and terminal multipolar phases. Prdm8 expression was almost coincident with Unc5D expression, a marker for the late multipolar phase, although the expression of Unc5D was found to be gradually down-regulated to the point at which mVenus expression was gradually up-regulated. This expression pattern suggests the possible involvement of Prdm8 in the control of the late and terminal multipolar phases, which controls the timing for morphological transition. To test this hypothesis, we performed gain- and loss-of-function analysis of neocortical development by using in utero electroporation. We found that the knockdown of Prdm8 results in premature change from multipolar to bipolar morphology, whereas the overexpression of Prdm8 maintained the multipolar morphology. Additionally, the postnatal analysis showed that the Prdm8 knockdown stimulated the number of early born neurons, and differentiated neurons located more deeply in the neocortex, however, majority of those cells could not acquire molecular features consistent with laminar location. Furthermore, we found the candidate genes that were predominantly utilized in both the late and terminal multipolar phases, and these candidate genes included those encoding for guidance molecules. In addition, we also found that the expression level of these guidance molecules was inhibited by the introduction of the Prdm8 expression vector. These results indicate that the Prdm8-mediated regulation of morphological changes that normally occur during the late and terminal multipolar phases plays an important role in neocortical development.
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