ArticlePloS one2014
The role of calpain-myosin 9-Rab7b pathway in mediating the expression of Toll-like receptor 4 in platelets: a novel mechanism involved in α-granules trafficking.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Functional toll-like receptor 4 links endotoxin sensing to platelet priming in feline platelets.Frontiers in veterinary science · 2025Article
- The innate immune response in SARS-CoV2 infection: focus on toll-like receptor 4 in severe disease outcomes.Frontiers in immunology · 2025Review
- Human pulmonary microvascular endothelial cells respond to DAMPs from injured renal tubular cells.Pulmonary circulation · 2024Article
- Interplay of TLR4 and SARS-CoV-2: Unveiling the Complex Mechanisms of Inflammation and Severity in COVID-19 Infections.Journal of inflammation research · 2024Review
- LL-37 Triggers Antimicrobial Activity in Human Platelets.International journal of molecular sciences · 2023Article
- Retinal Proteome Analysis Reveals a Region-Specific Change in the Rabbit Myopia Model.International journal of molecular sciences · 2023Article
- Thrombosis in COVID-19 infection: Role of platelet activation-mediated immunity.Thrombosis journal · 2021Review
- Effect of ultrapure lipopolysaccharides derived from diverse bacterial species on the modulation of platelet activation.Scientific reports · 2019Article
- The Role of Human Platelet Preparation for Toll-Like Receptors 2 and 4 Related Platelet Responsiveness.TH open : companion journal to thrombosis and haemostasis · 2019Article
- Article
- A Membrane Potential- and Calpain-Dependent Reversal of Caspase-1 Inhibition Regulates Canonical NLRP3 Inflammasome.Cell reports · 2018Article
- Review
- Defective Protein Catabolism in Atherosclerotic Vascular Inflammation.Frontiers in cardiovascular medicine · 2017Review
- Calpain-6 confers atherogenicity to macrophages by dysregulating pre-mRNA splicing.The Journal of clinical investigation · 2016Article
- Eotaxin-2 increased toll-like receptor 4 expression in endothelial cellsAmerican journal of translational research · 2016Article
- A novel interaction between Rab7b and actomyosin reveals a dual role in intracellular transport and cell migration.Journal of cell science · 2014Article
- Calpain activity and Toll-like receptor 4 expression in platelet regulate haemostatic situation in patients undergoing cardiac surgery and coagulation in mice.Mediators of inflammation · 2014Article
- Rab7b at the intersection of intracellular trafficking and cell migration.Communicative & integrative biologyArticle
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toll-like receptors (TLRs) plays a critical role in innate immunity. In 2004, Aslam R. and Shiraki R. first determined that murine and human platelets express functional TLRs. Additionally, Andonegui G. demonstrated that platelets express TLR4, which contributes to thrombocytopenia. However, the underlying mechanisms of TLR4 expression by platelets have been rarely explored until now. The aim of this study was to identify the mechanism of TLR4 expression underlying thrombin treatment. The human washed platelets were used in this study. According to flowcytometry and western blot analysis, the surface levels of TLR4 were significantly enhanced in thrombin-activated human platelets and decreased by TMB-8, calpeptin, and U73122, but not Y27632 (a Rho-associated protein kinase ROCK inhibitor) indicating that thrombin-mediated TLR4 expression was modulated by PAR/PLC pathway, calcium and calpain. Co-immunoprecipitation (co-IP) assay demonstrated that the interaction between TLR4 and myosin-9 (a substrate of calpain) was regulated by calpain; cleavage of myosin-9 enhanced TLR4 expression in thrombin treated platelets. Transmission electron microscope data indicated that human platelets used α-granules to control TLR4 expression; the co-IP experiment suggested that myosin-9 did not coordinate with Rab7b to negatively regulate TLR4 trafficking in thrombin treated platelets. In summary, phospholipase Cγ-calpain-myosin 9-Rab7b axis was responsible for the mechanism underlying the regulation of TLR4 containing α-granules trafficking in thrombin-stimulated platelets, which was involved in coagulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.