ArticlePloS one2014
Disruption of TgPHIL1 alters specific parameters of Toxoplasma gondii motility measured in a quantitative, three-dimensional live motility assay.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 77 citations in OpenAlex.
- Identification of UCB-9721 as a potent inhibitor of MyoA, the essential class XIV myosin motor of apicomplexan parasites.bioRxiv : the preprint server for biology · 2026Article
- Protein arginine methyltransferase 5 is essential for virulence in Toxoplasma gondii.Parasites & vectors · 2026Article
- Distinct motors, shared mechanics: unifying principles of microbial gliding.Journal of bacteriology · 2026Review
- Function and interactions of a protein bridge between the inner membrane complex and subpellicular microtubules inMolecular biology of the cell · 2025Article
- Motility-dependent processes inmSphere · 2025Article
- Submicrometre spatiotemporal characterization of the Toxoplasma adhesion strategy for gliding motility.Nature microbiology · 2024Article
- Motility-dependent processes inbioRxiv : the preprint server for biology · 2024Article
- Alveolin proteins in the Toxoplasma inner membrane complex form a highly interconnected structure that maintains parasite shape and replication.PLoS biology · 2024Article
- Emergent actin flows explain distinct modes of gliding motility.Nature physics · 2024Article
- The cortical microtubules of Toxoplasma gondii underlie the helicity of parasite movement.Journal of cell science · 2023Article
- MyosinA is a druggable target in the widespread protozoan parasite Toxoplasma gondii.PLoS biology · 2023Article
- Toxoplasma gondii Dissemination in the Brain Is Facilitated by Infiltrating Peripheral Immune Cells.mBio · 2022Article
- A circular zone of attachment to the extracellular matrix provides directionality to the motility ofeLife · 2022Article
- An apical protein, Pcr2, is required for persistent movement by the human parasite Toxoplasma gondii.PLoS pathogens · 2022Article
- Transcending Dimensions in Apicomplexan Research: from Two-Dimensional to Three-DimensionalMicrobiology and molecular biology reviews : MMBR · 2022Review
- Article
- Rotation and propulsion in 3D active chiral droplets.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes ofeLife · 2019Article
- Lattice Boltzmann methods and active fluids.The European physical journal. E, Soft matter · 2019Review
- Alveolar proteins stabilize cortical microtubules in Toxoplasma gondii.Nature communications · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
T. gondii uses substrate-dependent gliding motility to invade cells of its hosts, egress from these cells at the end of its lytic cycle and disseminate through the host organism during infection. The ability of the parasite to move is therefore critical for its virulence. T. gondii engages in three distinct types of gliding motility on coated two-dimensional surfaces: twirling, circular gliding and helical gliding. We show here that motility in a three-dimensional Matrigel-based environment is strikingly different, in that all parasites move in irregular corkscrew-like trajectories. Methods developed for quantitative analysis of motility parameters along the smoothed trajectories demonstrate a complex but periodic pattern of motility with mean and maximum velocities of 0.58 ± 0.07 µm/s and 2.01 ± 0.17 µm/s, respectively. To test how a change in the parasite's crescent shape might affect trajectory parameters, we compared the motility of Δphil1 parasites, which are shorter and wider than wild type, to the corresponding parental and complemented lines. Although comparable percentages of parasites were moving for all three lines, the Δphil1 mutant exhibited significantly decreased trajectory lengths and mean and maximum velocities compared to the parental parasite line. These effects were either partially or fully restored upon complementation of the Δphil1 mutant. These results show that alterations in morphology may have a significant impact on T. gondii motility in an extracellular matrix-like environment, provide a possible explanation for the decreased fitness of Δphil1 parasites in vivo, and demonstrate the utility of the quantitative three-dimensional assay for studying parasite motility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.