Evidence map›Paper›PMID 24489570›Full record

ArticleThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale2013

Evaluation of MRSASelect (™) chromogenic medium for the early detection of methicillin-resistant Staphylococcus aureus from blood cultures.

Kanchana Manickam, Andrew Walkty, Philippe Rs Lagacé-Wiens, Heather Adam, Barbara Swan, Brenda McAdam, Peter Pieroni, Michelle Alfa, James A Karlowsky

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In one paragraph

Article in The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kanchana ManickamMicrobiology, Diagnostic Services of Manitoba; ; Department of Medical Microbiology, Faculty of Medicine, University of Manitoba;
Andrew WalktyMicrobiology, Diagnostic Services of Manitoba; ; Department of Medical Microbiology, Faculty of Medicine, University of Manitoba; ; Department of Medicine, Health Sciences Centre, Winnipeg, Manitoba.
Philippe Rs Lagacé-WiensMicrobiology, Diagnostic Services of Manitoba; ; Department of Medical Microbiology, Faculty of Medicine, University of Manitoba;
Heather AdamMicrobiology, Diagnostic Services of Manitoba; ; Department of Medical Microbiology, Faculty of Medicine, University of Manitoba;
Barbara SwanMicrobiology, Diagnostic Services of Manitoba;
Brenda McAdamMicrobiology, Diagnostic Services of Manitoba;
Peter PieroniMicrobiology, Diagnostic Services of Manitoba;
Michelle AlfaMicrobiology, Diagnostic Services of Manitoba; ; Department of Medical Microbiology, Faculty of Medicine, University of Manitoba;
James A KarlowskyMicrobiology, Diagnostic Services of Manitoba; ; Department of Medical Microbiology, Faculty of Medicine, University of Manitoba;

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionStaphylococcus aureus bacteremia is associated with considerable morbidity and mortality. In theory, reducing the turnaround time in reporting of methicillin-resistant S aureus (MRSA) among patients with bactermia could assist with the rapid optimization of antimicrobial therapy.

objectiveTo evaluate the sensitivity and specificity of MRSASelect (Bio-Rad Laboratories, USA), a chromogenic medium, in the early detection of MRSA from blood cultures growing Gram-positive cocci in clusters, and to confirm that routine use of this medium would, in fact, reduce turnaround time for MRSA identification.

methodsThe present study was conducted at three microbiology laboratories in Manitoba. Between April 2010 and May 2011, positive blood cultures with Gram-positive cocci in clusters visualized on Gram stain were subcultured to both MRSASelect and routine media. MRSA isolates were identified using conventional microbiological methods from routine media and using growth with the typical colony morphology (pink colony) on MRSASelect medium.

resultsA total of 490 blood cultures demonstrating Gram-positive cocci in clusters on Gram stain were evaluated. S aureus was recovered from 274 blood cultures, with 51 S aureus isolates (51 of 274 [18.6%]) identified as MRSA. MRSASelect medium had a sensitivity of 98%, a specificity of 100%, a positive predictive value of 100% and a negative predictive value of 99.8% for the recovery and identification of MRSA directly from positive blood culture bottles. In addition, use of MRSASelect medium was found to improve turnaround time in the detection of MRSA by almost 24 h relative to conventional methods. DISCUSSION: These data support the utility of MRSASelect medium for the rapid identification of MRSA from positive blood cultures. Further clinical studies are warranted to determine whether the improvement in turnaround time will result in a measurable reduction in suboptimal antimicrobial therapy and/or improvement in patient outcome.

Indexed as

Blood culturesChromogenic agarMRSAStaphylococcus aureus

Identifiers

PMID24489570
PMCPMC3905011

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.