ArticlePlant molecular biology reporter2009
Evaluation of Candidate Reference Genes for Real-Time Quantitative PCR of Plant Samples Using Purified cDNA as Template.
Article in Plant molecular biology reporter, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Arabidopsis TGA256 Transcription Factors Suppress Salicylic-Acid-Induced Sucrose Starvation.Plants (Basel, Switzerland) · 2023Article
- Selection and Evaluation of Candidate Reference Genes for Quantitative Real-Time PCR in Aboveground Tissues and Drought Conditions inFrontiers in genetics · 2022Article
- Spatio-temporal selection of reference genes in the two congeneric species of Glycyrrhiza.Scientific reports · 2021Article
- Article
- Selection of Reference Genes for qRT-PCR Analysis in Medicinal PlantPlants (Basel, Switzerland) · 2020Article
- Identification of candidate reference genes for quantitative RT-PCR in Miscanthus sinensis subjected to various abiotic stresses.Molecular biology reports · 2020Article
- Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis inBioMed research international · 2020Article
- Article
- Selection and Validation of Reference Genes for mRNA Expression by Quantitative Real-Time PCR Analysis in Neolamarckia cadamba.Scientific reports · 2018Article
- Article
- Differential Subplastidial Localization and Turnover of Enzymes Involved in Isoprenoid Biosynthesis in Chloroplasts.PloS one · 2016Article
- Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression.Journal of visualized experiments : JoVE · 2015Article
- Transcriptome analysis of callus from Picea balfouriana.BMC genomics · 2014Article
- Selection of reliable reference genes for gene expression studies in the biofuel plant Jatropha curcas using real-time quantitative PCR.International journal of molecular sciences · 2013Article
- How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population.BMC molecular biology · 2013Article
- Selection of reliable reference genes for gene expression studies using real-time PCR in tung tree during seed development.PloS one · 2012Article
- Reference gene selection for quantitative real-time PCR normalization in Quercus suber.PloS one · 2012Article
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4 authors.
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Abstract
Quantitative real-time polymerase chain reaction (qRT-PCR) is a precise method to measure changes in gene transcript level. Accurate quantification requires careful RNA quality assessment, determination of primer efficiency, and selection of an appropriate reference gene. While many experimental procedures for these purposes have been described for mammalian samples, the direct application of these methods to plant samples often introduces unexpected experimental errors due to the complex and variable nature of the ribosomal RNA species present in typical plant extracts. In this paper, we report a simple procedure for the purification and quantification of complementary DNA (cDNA) after reverse transcriptase reactions by microcapillary electrophoresis. The use of purified cDNA allows template concentrations to be more accurately standardized for SYBR Green PCR reactions and increases amplification efficiencies so that these closely resemble those determined by the standard curve method. These advantages facilitate a more precise evaluation of the transcript levels of candidate reference genes under various experimental conditions without bias from differences in reverse transcriptase efficiency, template loading, or the presence of PCR inhibitors following reverse transcription. Using samples from
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