ArticleNucleic acids research2014
Antibiotic stress-induced modulation of the endoribonucleolytic activity of RNase III and RNase G confers resistance to aminoglycoside antibiotics in Escherichia coli.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Ultrasensitive RNase H activity detection using the transcription-based hybrid probe and CRISPR/cas12a signal amplifier.Frontiers in pharmacology · 2025Article
- Comparative Transcriptomic Analysis of Flagellar-Associated Genes in Salmonella Typhimurium and Its rnc Mutant.Journal of microbiology (Seoul, Korea) · 2024Article
- Transcript-specific selective translation by specialized ribosomes bearing genome-encoded heterogeneous rRNAs in V. vulnificus CMCP6.Journal of microbiology (Seoul, Korea) · 2022Article
- RNase III Participates in the Adaptation to Temperature Shock and Oxidative Stress inMicroorganisms · 2022Article
- Specialised ribosomes as versatile regulators of gene expression.RNA biology · 2022Review
- RNase III, Ribosome Biogenesis and Beyond.Microorganisms · 2021Review
- Uncovering a delicate balance between endonuclease RNase III and ribosomal protein S15 in E. coli ribosome assembly.Biochimie · 2021Article
- Trans-acting regulators of ribonuclease activity.Journal of microbiology (Seoul, Korea) · 2021Review
- Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression.PLoS pathogens · 2021Article
- Azithromycin resistance mutations inMicrobial genomics · 2020Article
- The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli.Scientific reports · 2019Article
- RNase G controls tpiA mRNA abundance in response to oxygen availability in Escherichia coli.Journal of microbiology (Seoul, Korea) · 2019Article
- Divergent rRNAs as regulators of gene expression at the ribosome level.Nature microbiology · 2019Article
- COLFrontiers in microbiology · 2019Article
- Article
- Regulation of Escherichia coli RNase III activity.Journal of microbiology (Seoul, Korea) · 2015Review
- Stability of the osmoregulated promoter-derived proP mRNA is posttranscriptionally regulated by RNase III in Escherichia coli.Journal of bacteriology · 2015Article
- The large ribosomal subunit protein L9 enables the growth of EF-P deficient cells and enhances small subunit maturation.PloS one · 2015Article
- Two tandem RNase III cleavage sites determine betT mRNA stability in response to osmotic stress in Escherichia coli.PloS one · 2014Article
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9 authors.
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Abstract
Here, we report a resistance mechanism that is induced through the modulation of 16S ribosomal RNA (rRNA) processing on the exposure of Escherichia coli cells to aminoglycoside antibiotics. We observed decreased expression levels of RNase G associated with increased RNase III activity on rng mRNA in a subgroup of E. coli isolates that transiently acquired resistance to low levels of kanamycin or streptomycin. Analyses of 16S rRNA from the aminoglycoside-resistant E. coli cells, in addition to mutagenesis studies, demonstrated that the accumulation of 16S rRNA precursors containing 3-8 extra nucleotides at the 5' terminus, which results from incomplete processing by RNase G, is responsible for the observed aminoglycoside resistance. Chemical protection, mass spectrometry analysis and cell-free translation assays revealed that the ribosomes from rng-deleted E. coli have decreased binding capacity for, and diminished sensitivity to, streptomycin and neomycin, compared with wild-type cells. It was observed that the deletion of rng had similar effects in Salmonella enterica serovar Typhimurium strain SL1344. Our findings suggest that modulation of the endoribonucleolytic activity of RNase III and RNase G constitutes a previously uncharacterized regulatory pathway for adaptive resistance in E. coli and related gram-negative bacteria to aminoglycoside antibiotics.
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