ArticleAnnals of botany2014
BcMF8, a putative arabinogalactan protein-encoding gene, contributes to pollen wall development, aperture formation and pollen tube growth in Brassica campestris.
Article in Annals of botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 89 citations in OpenAlex.
- Arabinogalactan Proteins in Pollen-Pistil Interactions: Molecular Regulators of Reproductive Barriers and Compatibility.International journal of molecular sciences · 2026Review
- Floral Characteristics and Reproductive Biology in Brazilian Melon Accessions: Insights from Commercial and Exotic Varieties.Plants (Basel, Switzerland) · 2025Article
- A review on the function of arabinogalactan-proteins during pollen grain development.Plant reproduction · 2025Review
- Gene expression profiles and metabolic pathways responsible for male sterility in cybrid pummelo.Plant cell reports · 2024Article
- Cluster effect through the oligomerisation of bioactive disaccharide AMOR on pollen tube capacitation inRSC chemical biology · 2024Article
- Heterologous Expression of TwoInternational journal of molecular sciences · 2023Article
- A splice site mutation in theHorticulture research · 2023Article
- The mutation of C-24 reductase, a key enzyme involved in brassinolide biosynthesis, confers a novel compact plant architecture phenotype to cucumber.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Arabinogalactan Proteins: Focus on the Role in Cellulose Synthesis and Deposition during Plant Cell Wall Biogenesis.International journal of molecular sciences · 2022Review
- OsFLA1 encodes a fasciclin-like arabinogalactan protein and affects pollen exine development in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- A Review of the Developmental Processes and Selective Pressures Shaping Aperture Pattern in Angiosperms.Plants (Basel, Switzerland) · 2022Review
- Highly Overexpressed AtC3H18 Impairs MicrogametogenesisFrontiers in plant science · 2022Article
- Hydroxyproline-Frontiers in plant science · 2022Article
- Article
- Global transcriptome dissection of pollen-pistil interactions induced self-incompatibility in dragon fruit (PeerJ · 2022Article
- The SlHB8 acts as a negative regulator in tapetum development and pollen wall formation in Tomato.Horticulture research · 2022Article
- Defect inFrontiers in plant science · 2022Article
- Comprehensive Analysis of Arabinogalactan Protein-Encoding Genes Reveals the Involvement of ThreeInternational journal of molecular sciences · 2021Article
- Building a Flower: The Influence of Cell Wall Composition on Flower Development and Reproduction.Genes · 2021Review
- FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis.BMC plant biology · 2021Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsThe arabinogalactan protein (AGP) gene family is involved in plant reproduction. However, little is known about the function of individual AGP genes in pollen development and pollen tube growth. In this study, Brassica campestris male fertility 8 (BcMF8), a putative AGP-encoding gene previously found to be pollen specific in Chinese cabbage (B. campestris ssp. chinensis), was investigated.
methodsReal-time reverse transcription-PCR and in situ hybridization were used to analyse the expression pattern of BcMF8 in pistils. Prokaryotic expression and western blots were used to ensure that BcMF8 could encode a protein. Antisense RNA technology was applied to silence gene expression, and morphological and cytological approaches (e.g. scanning electron microscopy and transmission electron microscopy) were used to reveal abnormal phenotypes caused by gene silencing. KEY
resultsThe BcMF8 gene encoded a putative AGP protein that was located in the cell wall, and was expressed in pollen grains and pollen tubes. The functional interruption of BcMF8 by antisense RNA technology resulted in slipper-shaped and bilaterally sunken pollen with abnormal intine development and aperture formation. The inhibition of BcMF8 led to a decrease in the percentage of in vitro pollen germination. In pollen that did germinate, the pollen tubes were unstable, abnormally shaped and burst more frequently relative to controls, which corresponded to an in vivo arrest of pollen germination at the stigma surface and retarded pollen tube growth in the stylar transmitting tissues.
conclusionsThe phenotypic defects of antisense BcMF8 RNA lines (bcmf8) suggest a crucial function of BcMF8 in modulating the physical nature of the pollen wall and in helping in maintaining the integrity of the pollen tube wall matrix.
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