ArticleMolecular biology reports2014
Agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf midribs as explants in ramie [Boehmeria nivea (L.) Gaud].
Article in Molecular biology reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Improving Ramie Fiber: Current Progress and Future Directions in Molecular Breeding.Plants (Basel, Switzerland) · 2026Review
- Chloroplast genome characteristics and phylogenetic analysis of Ramie (Boehmeria nivea (L.) Gaudich).Molecular biology reports · 2026Article
- Fipexide (FPX), a chemical callus inducer, promotes in vitro shoot regeneration andPlant biotechnology (Tokyo, Japan) · 2026Article
- Improved Protocol for EfficientPlants (Basel, Switzerland) · 2024Article
- Exploring Agrobacterium-mediated genetic transformation methods and its applications in Lilium.Plant methods · 2024Review
- Overexpression ofHeliyon · 2024Article
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- Elite hairy roots of3 Biotech · 2018Article
- Article
- Senescence is delayed when ramie (FEBS open bio · 2017Article
- A Genetic Transformation Method for Cadmium HyperaccumulatorFrontiers in plant science · 2017Article
- Optimization ofFrontiers in plant science · 2017Article
- Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Comparative Proteome Analysis of the Response of Ramie under Drought Stress.International journal of molecular sciences · 2016Article
- Transcriptome profiling and identification of transcription factors in ramie (Boehmeria nivea L. Gaud) in response to PEG treatment, using illumina paired-end sequencing technology.International journal of molecular sciences · 2015Article
- Isolation and characterization of the Agvip1 gene and response to abiotic and metal ions stresses in three celery cultivars.Molecular biology reports · 2014Article
- Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, leaf midribs, the elite explants, were used for the first time to develop an efficient regeneration and transformation protocol for ramie [Boehmeria nivea (L.) Gaud.] via Agrobacterium-mediated genetic transformation. Sensitivity of leaf midribs regeneration to kanamycin was evaluated, which showed that 40 mg l(-1) was the optimal concentration needed to create the necessary selection pressure. Factors affecting the ramie transformation efficiency were evaluated, including leaf age, Agrobacterium concentration, length of infection time for the Agrobacterium solution, acetosyringone concentration in the co-cultivation medium, and the co-cultivation period. The midrib explants from 40-day-old in vitro shoots, an Agrobacterium concentration at OD600 of 0.6, 10-min immersion in the bacteria solution, an acetosyringone concentration of 50 mg l(-1) in the co-cultivation medium and a 3-day co-cultivation period produced the highest efficiencies of regeneration and transformation. In this study, the average transformation rate was 23.25%. Polymerase chain reactions using GUS and NPTII gene-specific primers, Southern blot and histochemical GUS staining analyses further confirmed that the transgene was integrated into the ramie genome and expressed in the transgenic ramie. The establishment of this system of Agrobacterium-mediated genetic transformation and regeneration of transgenic plants will be used not only to introduce genes of interest into the ramie genome for the purpose of trait improvement, but also as a common means of testing gene function by enhancing or inhibiting the expression of target genes.
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