ArticleJournal of bacteriology2014
Roles of cyclic Di-GMP and the Gac system in transcriptional control of the genes coding for the Pseudomonas putida adhesins LapA and LapF.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 82 citations in OpenAlex.
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- Genetic basis of biofilm formation and salt adaptation in the plant-beneficial strain Stutzerimonas stutzeri MJL19.Applied microbiology and biotechnology · 2025Article
- A WYL domain transcription factor regulates Lactiplantibacillus plantarum intestinal colonization via perceiving c-di-GMP.Nature communications · 2025Article
- A Novel Membrane-Associated Protein Aids Bacterial Colonization of Maize.ACS synthetic biology · 2025Article
- Impact of fleQ Deficiency on Resource Allocation and Heterologous Gene Expression in Pseudomonas putida Across Various Growth Media.Microbial biotechnology · 2024Article
- Lignin conversion to β-ketoadipic acid byScience advances · 2023Article
- Adaption ofMicroorganisms · 2023Review
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- Nutrient Sensing and Biofilm Modulation: The Example of L-arginine inInternational journal of molecular sciences · 2022Review
- Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing.Applied and environmental microbiology · 2022Article
- Role of the Transcriptional Regulator ArgR in the Connection between Arginine Metabolism and c-di-GMP Signaling in Pseudomonas putida.Applied and environmental microbiology · 2022Article
- Article
- Bacterial extracellular matrix as a natural source of biotechnologically multivalent materials.Computational and structural biotechnology journal · 2021Review
- Adaptive laboratory evolution ofMetabolic engineering communications · 2020Article
- From Input to Output: The Lap/c-di-GMP Biofilm Regulatory Circuit.Annual review of microbiology · 2020Review
- MapA, a Second Large RTX Adhesin Conserved across the Pseudomonads, Contributes to Biofilm Formation by Pseudomonas fluorescens.Journal of bacteriology · 2020Article
- Arginine as an environmental and metabolic cue for cyclic diguanylate signalling and biofilm formation in Pseudomonas putida.Scientific reports · 2020Article
- Genetic factors involved in rhizosphere colonization by phytobeneficialComputational and structural biotechnology journal · 2020Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
LapA and LapF are large extracellular proteins that play a relevant role in biofilm formation by Pseudomonas putida. Current evidence favors a sequential model in which LapA is first required for the initial adhesion of individual bacteria to a surface, while LapF participates in later stages of biofilm development. In agreement with this model, lapF transcription was previously shown to take place at late times of growth and to respond to the stationary-phase sigma factor RpoS. We have now analyzed the transcription pattern of lapA and other regulatory elements that influence expression of both genes. The lapA promoter shows a transient peak of activation early during growth, with a second increase in stationary phase that is independent of RpoS. The same pattern is observed in biofilms although expression is not uniform in the population. Both lapA and lapF are under the control of the two-component regulatory system GacS/GacA, and their transcription also responds to the intracellular levels of the second messenger cyclic diguanylate (c-di-GMP), although in surprisingly reverse ways. Whereas expression from the lapA promoter increases with high levels of c-di-GMP, the opposite is true for lapF. The transcriptional regulator FleQ is required for the modulation of lapA expression by c-di-GMP but has a minor influence on lapF. This work represents a further step in our understanding of the regulatory interactions controlling biofilm formation in P. putida.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.