Evidence map›Paper›PMID 24487629›Full record

ArticleScientific reports2014

Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system.

Yicheng Zhao, Zhen Dai, Yang Liang, Ming Yin, Kuiying Ma, Mei He, Hongsheng Ouyang, Chun-Bo Teng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed.

  1. Review
  2. Review
  3. Therapeutic CRISPR epigenome editing of inflammatory receptors in the intervertebral disc.Molecular therapy : the journal of the American Society of Gene Therapy · 2024
    Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Synthetic biology tools for engineeringComputational and structural biotechnology journal · 2023
    Review
  9. Article
  10. Review
  11. Review
  12. Article
  13. Review
  14. Prokaryotic ncRNAs: Master regulators of gene expression.Current research in pharmacology and drug discovery · 2022
    Article
  15. Review
  16. Article
  17. Review
  18. Non-coding RNAs and potential therapeutic targeting in cancer.Biochimica et biophysica acta. Reviews on cancer · 2021
    Review
  19. Article
  20. CRISPR screen in cancer: status quo and future perspectives.American journal of cancer research · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yicheng Zhao1] College of life science, Northeast Forestry University, Harbin, China [2] Department of Biochemistry, College of Animal Sciences, Jilin University, China [3].
Zhen Dai1] Department of Biochemistry, College of Animal Sciences, Jilin University, China [2].
Yang Liang1] College of life science, Northeast Forestry University, Harbin, China [2].
Ming YinCollege of Veterinary Medicine, Jilin University, China.
Kuiying MaCollege of Veterinary Medicine, Jilin University, China.
Mei HeCollege of life science, Northeast Forestry University, Harbin, China.
Hongsheng OuyangDepartment of Biochemistry, College of Animal Sciences, Jilin University, China.
Chun-Bo TengCollege of life science, Northeast Forestry University, Harbin, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells.

Indexed as

3T3 CellsAnimalsBacterial ProteinsCell LineClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Associated Protein 9EndonucleasesGene ExpressionGene TargetingGreen Fluorescent ProteinsMiceMicroRNAsRecombinant Fusion ProteinsRNA, Guide, CRISPR-Cas SystemsRNA, Small InterferingSwineBacterial ProteinsCas9 protein, Francisella novicidaCRISPR-Associated Protein 9Endonucleasesenhanced green fluorescent proteinGreen Fluorescent ProteinsMicroRNAsRecombinant Fusion ProteinsRNA, Guide, CRISPR-Cas SystemsRNA, Small Interfering

Identifiers

PMID24487629
PMCPMC3909901

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.