ArticleApplied and environmental microbiology2014
Inactivation of human norovirus in contaminated oysters and clams by high hydrostatic pressure.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 56 citations in OpenAlex.
- The Effect of Non-Thermal Processing on the Fate of Pathogenic Bacteria and Hidden Hazardous Risks.Foods (Basel, Switzerland) · 2025Review
- PMAxx-RT-qPCR to Determine Human Norovirus Inactivation Following High-Pressure Processing of Oysters.Food and environmental virology · 2024Article
- Research Progress on Biological Accumulation, Detection and Inactivation Technologies of Norovirus in Oysters.Foods (Basel, Switzerland) · 2023Review
- Inactivation of Foodborne Viruses by High-Pressure Processing (HPP).Foods (Basel, Switzerland) · 2021Review
- Foodborne Viruses and Innovative Non-Thermal Food-Processing Technologies.Foods (Basel, Switzerland) · 2020Review
- Epidemiology of Foodborne Norovirus Outbreaks - United States, 2009-2015.Food safety (Tokyo, Japan) · 2018Article
- Critical Review on the Public Health Impact of Norovirus Contamination in Shellfish and the Environment: A UK Perspective.Food and environmental virology · 2017Review
- Variable High-Pressure-Processing Sensitivities for Genogroup II Human Noroviruses.Applied and environmental microbiology · 2016Article
- Modeling and Prediction of Oyster Norovirus Outbreaks along Gulf of Mexico Coast.Environmental health perspectives · 2016Article
- Thermal Inactivation of Feline Calicivirus in Pet Food Processing.Food and environmental virology · 2015Article
- Evaluation of the porcine gastric mucin binding assay for high-pressure-inactivation studies using murine norovirus and tulane virus.Applied and environmental microbiology · 2015Article
- Comprehensive comparison of cultivable norovirus surrogates in response to different inactivation and disinfection treatments.Applied and environmental microbiology · 2014Article
- High Pressure Processing of Bivalve Shellfish and HPP's Use as a Virus Intervention.Foods (Basel, Switzerland) · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human norovirus (NoV) is the most frequent causative agent of food-borne disease associated with shellfish consumption. In this study, the effect of high hydrostatic pressure (HHP) on inactivation of NoV was determined. Genogroup I.1 (GI.1) or genogroup II.4 (GII.4) NoV was inoculated into oyster homogenates and treated at 300 to 600 MPa at 25, 6, and 1°C for 5 min. After HHP, samples were treated with RNase and viral particles were extracted with porcine gastric mucin (PGM)-conjugated magnetic beads (PGM-MBs). Viral RNA was then quantified by real-time reverse transcription (RT)-PCR. Since PGM contains histo-blood group-like antigens, which can act as receptors for NoV, deficiency for binding to PGM is an indication of loss of infectivity of NoV. After binding to PGM-MBs, RT-PCR-detectable NoV RNA in oysters was reduced by 0.4 to >4 log10 by HHP at 300 to 600 MPa. The GI.1 NoV was more resistant to HHP than the GII.4 NoV (P < 0.05). HHP at lower temperatures significantly enhanced the inactivation of NoV in oysters (P < 0.05). Pressure treatment was also conducted for clam homogenates. Treatment at 450 MPa at 1°C achieved a >4 log10 reduction of GI.1 NoV in both oyster and clam homogenates. It is therefore concluded that HHP could be applied as a potential intervention for inactivating NoV in raw shellfish. The method of pretreatment of samples with RNase, extraction of viral particles using PGM-MB binding, and quantification of viral RNA using RT-PCR can be explored as a practical means of distinguishing between infectious and noninfectious NoV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.