ArticleApplied and environmental microbiology2014
RNA-stable-isotope probing shows utilization of carbon from inulin by specific bacterial populations in the rat large bowel.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Metagenomic and Metabolomic Signatures of Gut Microbiota AfterInternational journal of molecular sciences · 2026Observational
- From diet to function: using synthetic microbial communities to map gut microbial interactions.NPJ biofilms and microbiomes · 2026Review
- Classifying compounds as prebiotics - scientific perspectives and recommendations.Nature reviews. Gastroenterology & hepatology · 2025Review
- Exploring Bacterial Attributes That Underpin Symbiont Life in the Monogastric Gut.Applied and environmental microbiology · 2022Review
- Modulating the Gut Microbiota of Humans by Dietary Intervention with Plant Glycans.Applied and environmental microbiology · 2021Review
- Article
- Galacto- and Fructo-oligosaccharides Utilized for Growth by Cocultures of Bifidobacterial Species Characteristic of the Infant Gut.Applied and environmental microbiology · 2020Article
- Traditional Processed Meat Products Re-designed Towards Inulin-rich Functional Foods Reduce Polyps in Two Colorectal Cancer Animal Models.Scientific reports · 2019Article
- Bifidobacterium bifidum ATCC 15696 and Bifidobacterium breve 24b Metabolic Interaction Based on 2'-Applied and environmental microbiology · 2019Article
- Stable-Isotope Probing of Human and Animal Microbiome Function.Trends in microbiology · 2018Review
- Antibodies Set Boundaries Limiting Microbial Metabolite Penetration and the Resultant Mammalian Host Response.Immunity · 2018Article
- Bifidobacterium pseudolongum in the Ceca of Rats Fed Hi-Maize Starch Has Characteristics of a Keystone Species in Bifidobacterial Blooms.Applied and environmental microbiology · 2018Article
- Article
- Gut microbiota functions: metabolism of nutrients and other food components.European journal of nutrition · 2018Review
- Modulation of the Gastrointestinal Microbiome with Nondigestible Fermentable Carbohydrates To Improve Human Health.Microbiology spectrum · 2017Review
- Gastroparesis and lipid metabolism-associated dysbiosis in Wistar-Kyoto rats.American journal of physiology. Gastrointestinal and liver physiology · 2017Article
- Microbial nutrient niches in the gut.Environmental microbiology · 2017Review
- Dark Chocolate: Opportunity for an Alliance between Medical Science and the Food Industry?Frontiers in nutrition · 2017Article
- Determination of Resistant Starch Assimilating Bacteria in Fecal Samples of Mice byFrontiers in microbiology · 2017Article
- RNA-Based Stable Isotope Probing SuggestsBioMed research international · 2017Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knowledge of the trophisms that underpin bowel microbiota composition is required in order to understand its complex phylogeny and function. Stable-isotope ((13)C)-labeled inulin was added to the diet of rats on a single occasion in order to detect utilization of inulin-derived substrates by particular members of the cecal microbiota. Cecal digesta from Fibruline-inulin-fed rats was collected prior to (0 h) and at 6, 12, 18 and 24 h following provision of the [(13)C]inulin diet. RNA was extracted from these cecal specimens and fractionated in isopycnic buoyant density gradients in order to detect (13)C-labeled nucleic acid originating in bacterial cells that had metabolized the labeled dietary constituent. RNA extracted from specimens collected after provision of the labeled diet was more dense than 0-h RNA. Sequencing of 16S rRNA genes amplified from cDNA obtained from these fractions showed that Bacteroides uniformis, Blautia glucerasea, Clostridium indolis, and Bifidobacterium animalis were the main users of the (13)C-labeled substrate. Culture-based studies of strains of these bacterial species enabled trophisms associated with inulin and its hydrolysis products to be identified. B. uniformis utilized Fibruline-inulin for growth, whereas the other species used fructo-oligosaccharide and monosaccharides. Thus, RNA-stable-isotope probing (RNA-SIP) provided new information about the use of carbon from inulin in microbiota metabolism.
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Registered trials
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