ArticleNature medicine2014
Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe.
Article in Nature medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 105 citations in OpenAlex.
- Magneto-electrochemical biosensing for pathogen detection using nuclease-responsive nanohybrids.Mikrochimica acta · 2026Article
- Modulating Micrococcal Nuclease-Triggered Antibiotic Releases for One-Stage Revisions ofACS infectious diseases · 2026Article
- Next stop, precision: targeted therapies forFuture microbiology · 2025Article
- Mixed-Layered Glycodendrimer Probe for Imaging Inflammation at Surgical Site Infections.ACS sensors · 2025Article
- A fluorogenic micrococcal nuclease-based probe for fast detection and optical imaging of Staphylococcus aureus in prosthetic joint and fracture-related infections.European journal of nuclear medicine and molecular imaging · 2024Article
- Triggered Release of Ampicillin from Metallic Implant Coatings for Combating Periprosthetic Infections.ACS applied materials & interfaces · 2024Article
- Injectable Nano-Micro Composites with Anti-bacterial and Osteogenic Capabilities for Minimally Invasive Treatment of Osteomyelitis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- In Vivo Detection ofInternational journal of biomedical imaging · 2024Article
- Modulating On-Demand Release of Vancomycin from Implant Coatings via Chemical Modification of a Micrococcal Nuclease-Sensitive Oligonucleotide Linker.ACS applied materials & interfaces · 2023Article
- Liposome Delivery of Nucleic Acids in Bacteria: TowardACS infectious diseases · 2022Article
- Nucleases as molecular targets for cancer diagnosis.Biomarker research · 2021Review
- Ultra-Sensitive and Specific Detection ofDiagnostics (Basel, Switzerland) · 2021Article
- Rational Design and Experimental Analysis of Short-Oligonucleotide Substrate Specificity for Targeting Bacterial Nucleases.Journal of medicinal chemistry · 2021Article
- Advances in activity-based diagnostics for infectious disease and microbiome health.Current opinion in biomedical engineering · 2021Article
- Influence of sub-inhibitory concentrations of antimicrobials on micrococcal nuclease and biofilm formation in Staphylococcus aureus.Scientific reports · 2021Article
- Comparison of two fluorescent probes in preclinical non-invasive imaging and image-guided debridement surgery of Staphylococcal biofilm implant infections.Scientific reports · 2021Article
- Discovery and Proof-of-Concept Study of Nuclease Activity as a Novel Biomarker for Breast Cancer Tumors.Cancers · 2021Article
- Micrococcal Nuclease stimulatesFrontiers in cellular and infection microbiology · 2021Article
- Remote near infrared identification of pathogens with multiplexed nanosensors.Nature communications · 2020Article
- The smart activatable P2&3TT probe allows accurate, fast, and highly sensitive detection of Staphylococcus aureus in clinical blood culture samples.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Technologies that enable the rapid detection and localization of bacterial infections in living animals could address an unmet need for infectious disease diagnostics. We describe a molecular imaging approach for the specific, noninvasive detection of S. aureus based on the activity of the S. aureus secreted nuclease, micrococcal nuclease (MN). Several short synthetic oligonucleotides, rendered resistant to mammalian serum nucleases by various chemical modifications and flanked with a fluorophore and quencher, were activated upon degradation by purified MN and in S. aureus culture supernatants. A probe consisting of a pair of deoxythymidines flanked by several 2'-O-methyl-modified nucleotides was activated in culture supernatants of S. aureus but not in culture supernatants of several other pathogenic bacteria. Systemic administration of this probe to mice bearing S. aureus muscle infections resulted in probe activation at the infection sites in an MN-dependent manner. This new bacterial imaging approach has potential clinical applicability for infections with S. aureus and several other medically important pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.