ArticlePflugers Archiv : European journal of physiology1987
Effects of neomycin on calcium channel currents in clonal GH3 pituitary cells.
Article in Pflugers Archiv : European journal of physiology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 26 citations in OpenAlex.
- Block of the ryanodine receptor channel by neomycin is relieved at high holding potentials.Biophysical journal · 2002Article
- Gentamicin blocks ACh-evoked K+ current in guinea-pig outer hair cells by impairing Ca2+ entry at the cholinergic receptor.The Journal of physiology · 2000Article
- Block of single L-type Ca2+ channels in skeletal muscle fibers by aminoglycoside antibiotics.The Journal of general physiology · 1996Article
- Aminoglycoside antibiotics block voltage-dependent calcium channels in intact vertebrate nerve terminals.The Journal of general physiology · 1992Article
- Actions of neomycin on neuronal L-, N-, and non-L/non-N-type voltage-sensitive calcium channel responses.Journal of molecular neuroscience : MN · 1992Article
- Aminoglycoside blockade of Ca2(+)-activated K+ channel from rat brain synaptosomal membranes incorporated into planar bilayers.The Journal of membrane biology · 1990Article
- Effects of purinergic stimulation on the Ca current in single frog cardiac cells.Pflugers Archiv : European journal of physiology · 1990Article
- Currents carried by sodium ions through transient calcium channels in clonal GH3 pituitary cells.Pflugers Archiv : European journal of physiology · 1987Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane Ca currents were recorded from voltage-clamped clonal (GH3) pituitary cells under conditions where currents through Na and K channels were abolished. Two Ca currents, with distinct kinetics and voltage dependence for activation and inactivation, were identified. Neomycin, an aminoglycoside polycation, inhibited both the "transient" (ICa,t) and the "slowly-inactivating" (ICa,s) Ca currents in a dose-dependent manner (100-1,000 microM). The blockade was reversible and ICa,s was more sensitive to neomycin than ICa,t. The inhibition of ICa,s was frequency and time-independent, and was not affected by changes in the holding membrane potential (-35 to -100 mV). Neomycin did not affect the voltage dependence for inactivation of ICa,t. The blockade of both Ca currents by neomycin is ascribed to the general property of aminoglycosides to compete with, and displace Ca ions from membrane binding sites that determine the currents and selectivity of Ca channels. Because comparable concentrations of neomycin were required for blocking the currents conveyed by Ca or, in the absence of external divalent cations, by Na ions through the slowly-inactivating Ca channels, we suggest that the neomycin binding sites are distinct from the high-affinity transition sites within the Ca channel path.
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