ArticleCurrent biology : CB2014
A PIP5 kinase essential for efficient chemotactic signaling.
Article in Current biology : CB, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- PIP5K-Ras bistability triggers plasma membrane symmetry breaking to define cellular polarity and regulate migration.bioRxiv : the preprint server for biology · 2025Article
- The Amoebal Model for Macropinocytosis.Sub-cellular biochemistry · 2022Article
- Gradients of PI(4,5)PFrontiers in cell and developmental biology · 2022Article
- Three-dimensional stochastic simulation of chemoattractant-mediated excitability in cells.PLoS computational biology · 2021Article
- Review
- Excitable networks controlling cell migration during development and disease.Seminars in cell & developmental biology · 2020Review
- The Dual Role of Macropinocytosis in Cancers: Promoting Growth and Inducing Methuosis to Participate in Anticancer Therapies as Targets.Frontiers in oncology · 2020Review
- Wave patterns organize cellular protrusions and control cortical dynamics.Molecular systems biology · 2019Article
- Excitable dynamics of Ras triggers spontaneous symmetry breaking of PIP3 signaling in motile cells.Journal of cell science · 2019Article
- The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module.Developmental cell · 2019Article
- The origins and evolution of macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Phosphoinositide 3-kinase pathways and autophagy require phosphatidylinositol phosphate kinases.Advances in biological regulation · 2018Review
- Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum.PloS one · 2018Article
- Image based modeling of bleb site selection.Scientific reports · 2017Article
- Altering the threshold of an excitable signal transduction network changes cell migratory modes.Nature cell biology · 2017Article
- A polycystin-type transient receptor potential (Trp) channel that is activated by ATP.Biology open · 2017Article
- The Inositol-3-Phosphate Synthase Biosynthetic Enzyme Has Distinct Catalytic and Metabolic Roles.Molecular and cellular biology · 2016Article
- IQGAP1 is a phosphoinositide effector and kinase scaffold.Advances in biological regulation · 2016Review
- PIP kinases define PI4,5P₂signaling specificity by association with effectors.Biochimica et biophysica acta · 2015Review
- Dictyostelium uses ether-linked inositol phospholipids for intracellular signalling.The EMBO journal · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In neutrophils and Dictyostelium, chemoattractant gradients generate directed cell migration by eliciting signaling events that bias intrinsic motility and favor the production and retention of upgradient pseudopods. Phosphoinositides are actively regulated during chemotaxis in these cells, most iconically in the production of PI(3,4,5)P3 gradients within the plasma membrane. Although it is now known that PI(3,4,5)P3 signaling is nonessential for gradient sensing, the role of the related phosphoinositide PI(4,5)P2 is little understood, despite its clear importance in many cell biological processes. We describe here a PIP5 kinase, PikI, which produces PI(4,5)P2 and is essential for efficient chemotaxis of Dictyostelium cells. Without PikI, PI(4,5)P2 levels are reduced by 90%, and while pikI(-) cells move at normal speeds, they are highly disorientated in cAMP gradients. Following chemotactic stimulation, Ras is efficiently activated in pikI(-) cells, yet Ras-dependent responses (including activation of PKB) are severely impaired. PikI is phosphorylated by PKB, and in vitro studies of a phosphomimic mutant suggest that this phosphorylation increases PikI activity. We propose that adequate PI(4,5)P2 levels are required to couple activated Ras to its downstream effectors and that these levels are regulated by PikI, making it a crucial player in gradient sensing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.