ArticleBiomaterials2014
Spatial patterning of BMP-2 and BMP-7 on biopolymeric films and the guidance of muscle cell fate.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- A Review on Bone Tumor Management: Cutting-Edge Strategies in Bone Grafting, Bone Graft Substitute, and Growth Factors for Defect Reconstruction.Orthopedic research and reviews · 2025Review
- Recent Developments in Layer-by-Layer Assembly for Drug Delivery and Tissue Engineering Applications.Advanced healthcare materials · 2024Review
- Thickness Gradient in Polymer Coating by Reactive Layer-by-Layer Assembly on Solid Substrate.ACS omega · 2023Article
- Cellular Response to Bone Morphogenetic Proteins-2 and -7 Covalently Bound to Photocrosslinked Heparin-Diazoresin Multilayer.Biomolecules · 2023Article
- Unveiling the landscape of cytokine research in glioma immunotherapy: a scientometrics analysis.Frontiers in pharmacology · 2023Review
- Injectable stress relaxation gelatin-based hydrogels with positive surface charge for adsorption of aggrecan and facile cartilage tissue regeneration.Journal of nanobiotechnology · 2021Article
- Review
- Traction and attraction: haptotaxis substrates collagen and fibronectin interact with chemotaxis by HGF to regulate myoblast migration in a microfluidic device.American journal of physiology. Cell physiology · 2020Article
- Bu-M-P-ing Iron: How BMP Signaling Regulates Muscle Growth and Regeneration.Journal of developmental biology · 2020Review
- Bioengineered Systems and Designer Matrices That Recapitulate the Intestinal Stem Cell Niche.Cellular and molecular gastroenterology and hepatology · 2018Review
- Review
- Polyelectrolyte multilayers for bio-applications: recent advancements.IET nanobiotechnology · 2017Review
- Signal mingle: Micropatterns of BMP-2 and fibronectin on soft biopolymeric films regulate myoblast shape and SMAD signaling.Scientific reports · 2017Article
- The modulation of stem cell behaviors by functionalized nanoceramic coatings on Ti-based implants.Bioactive materials · 2016Review
- Tuning cellular responses to BMP-2 with material surfaces.Cytokine & growth factor reviews · 2016Review
- Patterning Biomaterials for the Spatiotemporal Delivery of Bioactive Molecules.Frontiers in bioengineering and biotechnology · 2016Review
- Spatio-Temporal Control of LbL Films for Biomedical Applications: From 2D to 3D.Advanced healthcare materials · 2015Review
- Porous deproteinized bovine bone scaffold with three-dimensional localized drug delivery system using chitosan microspheres.Biomedical engineering online · 2015Article
- Surface-Bound Molecular Gradients for the High-Throughput Screening of Cell Responses.Frontiers in bioengineering and biotechnology · 2015Review
- Matrix-Immobilized BMP-2 on Microcontact Printed Fibronectin as an in vitro Tool to Study BMP-Mediated Signaling and Cell Migration.Frontiers in bioengineering and biotechnology · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
In the cellular microenvironment, growth factor gradients are crucial in dictating cell fate. Towards developing materials that capture the native microenvironment we engineered biomimetic films that present gradients of matrix-bound bone morphogenetic proteins (BMP-2 and BMP-7). To this end layer-by-layer films composed of poly(L-lysine) and hyaluronan were combined in a simple microfluidic device enabling spatially controlled growth factor diffusion along the film. Linear long-range gradients of both BMPs induced the trans-differentiation of C2C12 myoblasts towards the osteogenic lineage in a dose dependent manner with a different signature for each BMP. The osteogenic marker alkaline phosphatase (ALP) increased in a linear manner for BMP-7 and non-linearly for BMP-2. Moreover, an increased expression of the myogenic marker troponin T was observed with decreasing matrix-bound BMP concentration, providing a substrate that it is both osteo- and myo-inductive. Lastly, dual parallel matrix-bound gradients of BMP-2 and -7 revealed a complete saturation of the ALP signal. This suggested an additive or synergistic effect of the two BMPs. This simple technology allows for determining quickly and efficiently the optimal concentration of matrix-bound growth factors, as well as for investigating the presentation of multiple growth factors in their solid-phase and in a spatially controlled manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.