Evidence map›Paper›PMID 24485456›Full record

ArticleCell2014

Extracellular architecture of the SYG-1/SYG-2 adhesion complex instructs synaptogenesis.

Engin Özkan, Poh Hui Chia, Ruiqi Rachel Wang, Natalia Goriatcheva, Dominika Borek, Zbyszek Otwinowski, Thomas Walz, Kang Shen, K Christopher Garcia

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Structural Insights into Ordered Multicomponent Assemblies in Cell Junctions.Advances in experimental medicine and biology · 2026
    Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Review
  16. Article
  17. Structure and evolution of neuronal wiring receptors and ligands.Developmental dynamics : an official publication of the American Association of Anatomists · 2023
    Review
  18. Article
  19. Solution Structure of theFrontiers in cellular and infection microbiology · 2022
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Engin ÖzkanDepartment of Molecular and Cellular Physiology and Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
Poh Hui ChiaDepartment of Biology, Stanford University, Stanford, CA 94305, USA.
Ruiqi Rachel WangDepartment of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Natalia GoriatchevaDepartment of Molecular and Cellular Physiology and Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
Dominika BorekDepartments of Biochemistry and Biophysics, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
Zbyszek OtwinowskiDepartments of Biochemistry and Biophysics, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
Thomas WalzDepartment of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.
Kang ShenHoward Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Biology, Stanford University, Stanford, CA 94305, USA.
K Christopher GarciaDepartment of Molecular and Cellular Physiology and Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: kcgarcia@stanford.edu.

Funding

X-RAY CRYSTALLOGRAPHY OF MACROMOLECULESR01GM053163 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI MINOR, WLADEK, OTWINOWSKI, ZBYSZEK · 1996 to 2014
$6.3M
Mechanisms of Synaptic Specificity in C. elegansR01NS048392 · NINDS · STANFORD UNIVERSITY · PI SHEN, KANG · 2005 to 2014
$3.1M
Howard Hughes Medical InstituteNIGMS NIH HHS 5 R01 GM053163NIGMS NIH HHS R01 GM053163NINDS NIH HHS 5 R01 NS048392NINDS NIH HHS R01 NS048392
6 · The paper itself

Abstract

SYG-1 and SYG-2 are multipurpose cell adhesion molecules (CAMs) that have evolved across all major animal taxa to participate in diverse physiological functions, ranging from synapse formation to formation of the kidney filtration barrier. In the crystal structures of several SYG-1 and SYG-2 orthologs and their complexes, we find that SYG-1 orthologs homodimerize through a common, bispecific interface that similarly mediates an unusual orthogonal docking geometry in the heterophilic SYG-1/SYG-2 complex. C. elegans SYG-1's specification of proper synapse formation in vivo closely correlates with the heterophilic complex affinity, which appears to be tuned for optimal function. Furthermore, replacement of the interacting domains of SYG-1 and SYG-2 with those from CAM complexes that assume alternative docking geometries or the introduction of segmental flexibility compromised synaptic function. These results suggest that SYG extracellular complexes do not simply act as "molecular velcro" and that their distinct structural features are important in instructing synaptogenesis. PAPERFLICK:

Indexed as

Amino Acid SequenceAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsCell AdhesionDimerizationImmunoglobulinsModels, MolecularMolecular Sequence DataNerve Tissue ProteinsNeuronsProtein Structure, TertiarySequence AlignmentSynapsesCaenorhabditis elegans ProteinsImmunoglobulinsNerve Tissue ProteinsSYG-1 protein, C elegansSYG-2 protein, C elegans

Identifiers

PMID24485456
PMCPMC3962013

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.