ArticleGenome biology2014
Genome-wide co-localization of Polycomb orthologs and their effects on gene expression in human fibroblasts.
Article in Genome biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- A specific form of cPRC1 containing CBX4 is co-opted to mediate oncogenic gene repression in diffuse midline glioma.Molecular cell · 2025Article
- The Impact of Polycomb Group Proteins on 3D Chromatin Structure and Environmental Stresses in Plants.Plants (Basel, Switzerland) · 2025Review
- CBX7 reprograms metabolic flux to protect against meningioma progression by modulating the USP44/c-MYC/LDHA axis.Journal of molecular cell biology · 2024Article
- DNA elements tether canonical Polycomb Repressive Complex 1 to human genes.Nucleic acids research · 2023Article
- Context-specific Polycomb mechanisms in development.Nature reviews. Genetics · 2022Review
- Polycomb repressive complex 2 shields naïve human pluripotent cells from trophectoderm differentiation.Nature cell biology · 2022Article
- A Potent, Selective CBX2 Chromodomain Ligand and Its Cellular Activity During Prostate Cancer Neuroendocrine Differentiation.Chembiochem : a European journal of chemical biology · 2021Article
- The roles of Polycomb repressive complexes in mammalian development and cancer.Nature reviews. Molecular cell biology · 2021Review
- Phase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activity.Nature communications · 2020Article
- Functions of Polycomb Proteins on Active Targets.Epigenomes · 2020Review
- Dynamic Competition of Polycomb and Trithorax in Transcriptional Programming.Annual review of biochemistry · 2020Review
- Optimization of Ligands Using Focused DNA-Encoded Libraries To Develop a Selective, Cell-Permeable CBX8 Chromodomain Inhibitor.ACS chemical biology · 2020Article
- Polycomb Assemblies Multitask to Regulate Transcription.Epigenomes · 2019Review
- Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives chromatin association.Nucleic acids research · 2019Article
- The Polycomb group protein CBX6 is an essential regulator of embryonic stem cell identity.Nature communications · 2017Article
- Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes inGenes & development · 2017Article
- Demethylated HSATII DNA and HSATII RNA Foci Sequester PRC1 and MeCP2 into Cancer-Specific Nuclear Bodies.Cell reports · 2017Article
- Article
- CBX8 Suppresses Tumor Metastasis via Repressing Snail in Esophageal Squamous Cell Carcinoma.Theranostics · 2017Article
- Coordinate redeployment of PRC1 proteins suppresses tumor formation during Drosophila development.Nature genetics · 2016Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolycomb group proteins form multicomponent complexes that are important for establishing lineage-specific patterns of gene expression. Mammalian cells encode multiple permutations of the prototypic Polycomb repressive complex 1 (PRC1) with little evidence for functional specialization. An aim of this study is to determine whether the multiple orthologs that are co-expressed in human fibroblasts act on different target genes and whether their genomic location changes during cellular senescence.
resultsDeep sequencing of chromatin immunoprecipitated with antibodies against CBX6, CBX7, CBX8, RING1 and RING2 reveals that the orthologs co-localize at multiple sites. PCR-based validation at representative loci suggests that a further six PRC1 proteins have similar binding patterns. Importantly, sequential chromatin immunoprecipitation with antibodies against different orthologs implies that multiple variants of PRC1 associate with the same DNA. At many loci, the binding profiles have a distinctive architecture that is preserved in two different types of fibroblast. Conversely, there are several hundred loci at which PRC1 binding is cell type-specific and, contrary to expectations, the presence of PRC1 does not necessarily equate with transcriptional silencing. Interestingly, the PRC1 binding profiles are preserved in senescent cells despite changes in gene expression.
conclusionsThe multiple permutations of PRC1 in human fibroblasts congregate at common rather than specific sites in the genome and with overlapping but distinctive binding profiles in different fibroblasts. The data imply that the effects of PRC1 complexes on gene expression are more subtle than simply repressing the loci at which they bind.
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