ArticleBiomaterials2014
Quantitative characterization of mineralized silk film remodeling during long-term osteoblast-osteoclast co-culture.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Osteoblast-osteoclast co-cultures: A systematic review and map of available literature.PloS one · 2021Pooled it
- An Exploration of the Role of Osteoclast Lineage Cells in Bone Tissue Engineering.Tissue engineering. Part B, Reviews · 2025Review
- Developing a Bone-Mimicking Microenvironment: Surface Coating Method for Investigating Bone RemodelingACS biomaterials science & engineering · 2025Article
- Silk-Soy Alloy Materials: Influence of Silk Types (Mori, Thai, Muga, Tussah, and Eri) on the Structure, Properties, and Functionality of Insect-Plant Protein Blends (II).International journal of molecular sciences · 2025Article
- Biomaterial Cues for Regulation of Osteoclast Differentiation and Function in Bone Regeneration.Advanced therapeutics · 2025Article
- Concentric-mineralized hybrid silk-based scaffolds for bone tissue engineeringJournal of materials chemistry. B · 2023Article
- Tuning the resorption-formation balance in anBone reports · 2023Article
- Aspiration-assisted freeform bioprinting of mesenchymal stem cell spheroids within alginate microgels.Biofabrication · 2022Article
- Nanovibrational stimulation inhibits osteoclastogenesis and enhances osteogenesis in co-cultures.Scientific reports · 2021Article
- Zinc Sulfate Stimulates Osteogenic Phenotypes in Periosteum-Derived Cells and Co-Cultures of Periosteum-Derived Cells and THP-1 Cells.Life (Basel, Switzerland) · 2021Article
- Protein and Polysaccharide-Based Fiber Materials Generated from Ionic Liquids: A Review.Molecules (Basel, Switzerland) · 2020Review
- A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications.International journal of molecular sciences · 2020Review
- Bone physiology as inspiration for tissue regenerative therapies.Biomaterials · 2018Review
- Optimization of the Static Human Osteoblast/Osteoclast Co-culture System.Iranian journal of medical sciences · 2018Article
- Review
- Ice-Templated Protein Nanoridges Induce Bone Tissue Formation.Advanced functional materials · 2017Article
- Effect of serum from postmenopausal women with osteoporosis exhibiting the Kidney-Yang deficiency pattern on bone formation in an hFOB 1.19 human osteoblastic cell line.Experimental and therapeutic medicine · 2015Article
- Strategies for improving the physiological relevance of human engineered tissues.Trends in biotechnology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The goal of this study was to explore quantitative assessments of mineralized silk protein biomaterial films by co-cultures of human mesenchymal stem cell-derived osteoblasts and human acute monocytic leukemia cell line-derived osteoclasts during long-term culture (8-32 weeks). The remodeled films were quantitatively assessed using three different techniques during this extended cultivation to provide more comprehensive insight into the impact of co-cultures on surface remodeling. Scanning electron microscopy (SEM) with three dimensional surface reconstructions was used to quantitatively determine various surface morphological features and measures of roughness indicative of remodeling by the cells. Additionally, reconstructed surfaces were converted to depth images for Fourier analysis to quantify the potential fractal organization of biomineralization. The long-term remodeled films were also imaged using confocal reflectance microscopy and micro-computed tomography (micro-CT) to further quantify morphological changes. Films remodeled in co-culture demonstrated increased roughness parameters, fractal organization, and volume compared to films remodeled by osteoblasts alone. The combination of these techniques to quantify remodeling of mineralized protein films shows promise for quantifying processes related to mineralized surfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.