ArticleNature protocols2014
One-step targeted gene deletion in Candida albicans haploids.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- Sdd3 regulates the biofilm formation ofmBio · 2025Article
- Rapid evolution of an adaptive multicellular morphology of Candida auris during systemic infection.Nature communications · 2024Article
- Fungal resilience and host-pathogen interactions: Future perspectives and opportunities.Parasite immunology · 2023Review
- Comprehensive Interactome Analysis for the Sole Adenylyl Cyclase Cyr1 of Candida albicans.Microbiology spectrum · 2022Article
- Inactivating the mannose-ethanolamine phosphotransferase Gpi7 confers caspofungin resistance in the human fungal pathogenCell surface (Amsterdam, Netherlands) · 2021Article
- Orchestrated actin nucleation by theThe Journal of biological chemistry · 2020Article
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- Gene Essentiality Analyzed bymBio · 2018Article
- Candida albicans gains azole resistance by altering sphingolipid composition.Nature communications · 2018Article
- Maize Transposable ElementsG3 (Bethesda, Md.) · 2018Article
- Sac7 and Rho1 regulate the white-to-opaque switching in Candida albicans.Scientific reports · 2018Article
- Use of Haploid Model ofFrontiers in cellular and infection microbiology · 2018Article
- Parasex Generates Phenotypic DiversityGenetics · 2017Article
- Bacterial GtfB Augments Candida albicans Accumulation in Cross-Kingdom Biofilms.Journal of dental research · 2017Article
- Comparative Ploidy Proteomics of Candida albicans Biofilms Unraveled the Role of the AHP1 Gene in the Biofilm Persistence Against Amphotericin B.Molecular & cellular proteomics : MCP · 2016Article
- New "haploid biofilm model" unravels IRA2 as a novel regulator of Candida albicans biofilm formation.Scientific reports · 2015Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent discovery of haploids in Candida albicans and the construction of tool strains carrying multiple auxotrophic markers have enabled, for the first time, performing one-step gene deletions in this fungal human pathogen. This breakthrough promises to greatly facilitate the molecular and genetic study of C. albicans biology and pathogenicity. However, the construction of gene-deletion mutants in C. albicans haploids involves many technical difficulties, particularly low transformation efficiency and autodiploidization. Here we describe a highly effective protocol for designing and performing one-step gene deletion in C. albicans haploids, which takes ∼11 d to complete (not including plasmid construction, which may take ∼2 weeks). A gene deletion cassette is constructed on a plasmid and subsequently released for transformation by lithium acetate incubation or electroporation. Desired gene-deletion mutants are identified and their ploidy is assessed simultaneously by colony PCR before final confirmation by flow cytometry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.