ArticleIntegrative biology : quantitative biosciences from nano to macro2014
Ligand density elicits a phenotypic switch in human neutrophils.
Article in Integrative biology : quantitative biosciences from nano to macro, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Mechanisms of frustrated phagocytic spreading of human neutrophils on antibody-coated surfaces.Biophysical journal · 2022Article
- Integrative experimental/computational approach establishes active cellular protrusion as the primary driving force of phagocytic spreading by immune cells.PLoS computational biology · 2022Article
- Amoeboid Swimming Is Propelled by Molecular Paddling in Lymphocytes.Biophysical journal · 2020Article
- Gelatin-Based Microribbon Hydrogels Support Robust MSC Osteogenesis across a Broad Range of Stiffness.ACS biomaterials science & engineering · 2020Article
- Interaction with an endothelial lumen increases neutrophil lifetime and motility in response toBlood · 2018Article
- Edges impose planar alignment in nematic monolayers by directing cell elongation and enhancing migration.Soft matter · 2018Article
- Motile Dendritic Cells Sense and Respond to Substrate Geometry.Annals of biomedical engineering · 2018Article
- Computational study of cell adhesion and rolling in flow channel by meshfree method.Computer methods in biomechanics and biomedical engineering · 2017Article
- Strain and rate-dependent neuronal injury in a 3D in vitro compression model of traumatic brain injury.Scientific reports · 2016Article
- Motile Human Neutrophils Sense Ligand Density Over Their Entire Contact Area.Annals of biomedical engineering · 2016Article
- Time series modeling of live-cell shape dynamics for image-based phenotypic profiling.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- Protrusive and Contractile Forces of Spreading Human Neutrophils.Biophysical journal · 2015Article
- Effect of adhesion and chemokine presentation on T-lymphocyte haptokinesis.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Two-dimensional motility of a macrophage cell line on microcontact-printed fibronectin.Cytoskeleton (Hoboken, N.J.) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Neutrophils are mediators of innate immunity and motility is critical to their function. We used microcontact printing to investigate the relationship between density of adhesive ligands and the dynamics of neutrophil motility. We show that neutrophils adopt a well-spread morphology without a uropod on moderate densities of adhesion ligand. As density is increased, the morphology switches to a classic amoeboid shape. In addition to the morphological differences, the dynamics of motility were quantitatively distinct. Well-spread cells without uropods glide slowly with high persistence, while amoeboid cells made frequent directional changes migrating quickly with low persistence. Using an antibody panel against various integrin chains, we show that adhesion and motility on fibronectin are mediated by MAC-1 (αMβ2). The phenotypic switch could be generalized to other surface ligands, such as bovine serum albumin, to which the promiscuous MAC-1 also binds. These results suggest that neutrophils are capable of displaying multiple modes of motility as dictated by their adhesive environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.