ArticleJournal of biotechnology2014
28S rRNA is inducibly pseudouridylated by the mTOR pathway translational control in CHO cell cultures.
Article in Journal of biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Deciphering the pseudouridine nucleobase modification in human diseases: From molecular mechanisms to clinical perspectives.Clinical and translational medicine · 2025Review
- Subverting the Canon: Novel Cancer-Promoting Functions and Mechanisms for snoRNAs.International journal of molecular sciences · 2024Review
- Men who inject opioids exhibit altered tRNA-Gly-GCC isoforms in semen.Molecular human reproduction · 2023Article
- Pseudouridine as a novel biomarker in prostate cancer.Urologic oncology · 2021Review
- Regulation and Function of RNA Pseudouridylation in Human Cells.Annual review of genetics · 2020Review
- HydraPsiSeq: a method for systematic and quantitative mapping of pseudouridines in RNA.Nucleic acids research · 2020Article
- H/ACA snoRNA levels are regulated during stem cell differentiation.Nucleic acids research · 2020Article
- A dual role of the ribosome-bound chaperones RAC/Ssb in maintaining the fidelity of translation termination.Nucleic acids research · 2019Article
- Targeting snoRNAs as an emerging method of therapeutic development for cancer.American journal of cancer research · 2019Review
- Turning Uridines around: Role of rRNA Pseudouridylation in Ribosome Biogenesis and Ribosomal Function.Biomolecules · 2018Review
- Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion.Scientific reports · 2017Article
- Transcriptome-Wide Identification of Pseudouridine Modifications Using Pseudo-seq.Current protocols in molecular biology · 2015Article
- Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome.Nature chemical biology · 2015Article
- Pseudo-Seq: Genome-Wide Detection of Pseudouridine Modifications in RNA.Methods in enzymology · 2015Article
- Posttranscriptional modification of messenger RNAs in eukaryotes.Molecular biology · 2015Review
- An expanding universe of mRNA modifications.Nature structural & molecular biology · 2014Article
- Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA.Cell · 2014Article
- Pseudouridine: still mysterious, but never a fake (uridine)!RNA biology · 2014Review
- The emerging landscape of small nucleolar RNAs in cell biology.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
The mTOR pathway is a conserved master regulator of translational activity that influences the fate of industrially relevant CHO cell cultures, yet its molecular mechanisms remain unclear. Interestingly, rapamycin specific inhibition of the mTOR pathway in CHO cells was found to down-regulate the small nucleolar RNA U19 (snoRNA U19) by 2-fold via translatome profiling. snoRNA U19 guides the two most conserved pseudouridylation modifications on 28S ribosomal RNA (rRNA) that are important for the biogenesis and proper function of ribosomes. In order to further understand the role of snoRNA U19 as a potential player in the mTOR pathway, we measured 28S rRNA pseudouridylation upon rapamycin treatments and/or snoRNA U19 overexpression conditions, thereby characterizing the subsequent effects on ribosome efficiency and global translation by polysome profiling. We showed that 28S rRNA pseudouridylation was increased by rapamycin treatment and/or overexpression of snoRNA U19, but only the latter condition improved ribosome efficiency toward higher global translation, thus implying that the mTOR pathway induces pseudouridylation at different sites along the 28S rRNA possibly with either positive or negative effects on the cellular phenotype. This discovery of snoRNA U19 as a new downstream effector of the mTOR pathway suggests that cell engineering of snoRNAs can be used to regulate translation and improve cellular growth in CHO cell cultures in the future.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.