ArticleFrontiers in microbiology2013
Widespread dissemination of class 1 integron components in soils and related ecosystems as revealed by cultivation-independent analysis.
Article in Frontiers in microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 89 citations in OpenAlex.
- Stewarding the hospital sink drain: a narrative review of practical approaches for controlling gram negative pathogens in low- and middle-income countries.Antimicrobial resistance and infection control · 2026Review
- Promotion of plasmid maintenance by heterogeneous partitioning of microbial communities.Cell systems · 2023Article
- Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta.Microorganisms · 2023Article
- Development of a high-throughput platform to measure plasmid transfer frequency.Frontiers in cellular and infection microbiology · 2023Article
- Incidence of antibiotic resistance genotypes of Vibrio species recovered from selected freshwaters in Southwest Nigeria.Scientific reports · 2022Article
- Sequencing Depth Has a Stronger Effect than DNA Extraction on Soil Bacterial Richness Discovery.Biomolecules · 2022Article
- Distribution and Molecular Characterization of Functional Class 2 Integrons in ClinicalInfection and drug resistance · 2022Article
- Effect of biochar on antibiotics and antibiotic resistance genes variations during co-composting of pig manure and corn straw.Frontiers in bioengineering and biotechnology · 2022Article
- Existing evidence on antibiotic resistance exposure and transmission to humans from the environment: a systematic map.Environmental evidence · 2022Article
- Antibiotic Resistance in Agricultural Soil and Crops Associated to the Application of Cow Manure-Derived Amendments From Conventional and Organic Livestock Farms.Frontiers in veterinary science · 2021Article
- Evolution of antibiotic resistance at low antibiotic concentrations including selection below the minimal selective concentration.Communications biology · 2020Article
- Article
- Evaluation of antibiotic resistance dissemination by wastewater treatment plant effluents with different catchment areas in Germany.Scientific reports · 2020Article
- Article
- Plant Species-Dependent Increased Abundance and Diversity of IncP-1 Plasmids in the Rhizosphere: New Insights Into Their Role and Ecology.Frontiers in microbiology · 2020Article
- The Transferable Resistome of Produce.mBio · 2018Article
- Diversification of broad host range plasmids correlates with the presence of antibiotic resistance genes.FEMS microbiology ecology · 2016Article
- Integron diversity in marine environments.Environmental science and pollution research international · 2015Review
- Mining microbial metatranscriptomes for expression of antibiotic resistance genes under natural conditions.Scientific reports · 2015Article
- Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution.The ISME journal · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Class 1 integrons contribute to the emerging problem of antibiotic resistance in human medicine by acquisition, exchange, and expression of resistance genes embedded within gene cassettes. Besides the clinical setting they were recently reported from environmental habitats and often located on plasmids and transposons, facilitating their transfer and spread within bacterial communities. In this study we aimed to provide insights into the occurrence of genes typically associated with the class 1 integrons in previously not studied environments with or without human impact and their association with IncP-1 plasmids. Total community DNA was extracted from manure-treated and untreated soils, lettuce and potato rhizosphere, digestates, and an on-farm biopurification system and screened by PCR with subsequent Southern blot hybridization for the presence of the class 1 integrase gene intI1 as well as qacE and qacEΔ 1 resistance genes. The results revealed a widespread dissemination of class 1 integrons in the environments analyzed, mainly related to the presence of qacEΔ 1 genes. All 28 IncP-1ε plasmids carrying class 1 integrons, which were captured exogenously in a recent study from piggery manure and soils treated with manure, carried qacEΔ 1 genes. Based on the strong hybridization signals in the rhizosphere of lettuce compared to the potato rhizosphere, the abundances of intI1, qacE/qacEΔ 1, and sul1 genes were quantified relative to the 16S rRNA gene abundance by real-time PCR in the rhizosphere of lettuce planted in three different soils and in the corresponding bulk soil. A significant enrichment of intI1 and qacE/qacEΔ 1 genes was confirmed in the rhizosphere of lettuce compared to bulk soil. Additionally, the relative abundance of korB genes specific for IncP-1 plasmids was enriched in the rhizosphere and correlated to the intI1 gene abundance indicating that IncP-1 plasmids might have contributed to the spread of class 1 integrons in the analyzed soils.
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