ArticleJournal of clinical microbiology2014
Multilocus sequence typing of an emerging Cryptosporidium hominis subtype in the United States.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- A Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Scheme for Epidemiological Investigations of Cryptosporidium parvum.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Cryptosporidium Genotyping for Epidemiology Tracking.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Development and application of a novel beta-tubulin genotyping tool reveals host-specific transmission cluster in Balantioides coli.PLoS neglected tropical diseases · 2025Article
- Article
- Molecular identification and genetic characteristics of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in human immunodeficiency virus/acquired immunodeficiency syndrome patients in Shanghai, China.Parasites & vectors · 2023Article
- Multiple introductions and recombination events underlie the emergence of a hyper-transmissible Cryptosporidium hominis subtype in the USA.Cell host & microbe · 2023Article
- Global Population Genomics of Two Subspecies of Cryptosporidium hominis during 500 Years of Evolution.Molecular biology and evolution · 2022Article
- Article
- Development and Application of aMicroorganisms · 2021Article
- Review
- Article
- Comparative genomics: how has it advanced our knowledge of cryptosporidiosis epidemiology?Parasitology research · 2019Review
- Analysis of the Cryptosporidium spp. and gp60 subtypes linked to human outbreaks of cryptosporidiosis in England and Wales, 2009 to 2017.Parasites & vectors · 2019Article
- Direct Sequencing ofFrontiers in public health · 2019Review
- Differential Expression of ThreeFrontiers in microbiology · 2019Article
- Diverse single-amino-acid repeat profiles in the genus Cryptosporidium.Parasitology · 2018Article
- First detection of Cryptosporidium DNA in blood and cerebrospinal fluid of HIV-infected patients.Parasitology research · 2018Article
- Genomic Variation in IbA10G2 and Other Patient-Derived Cryptosporidium hominis Subtypes.Journal of clinical microbiology · 2017Article
- Article
- Molecular Epidemiology of Cryptosporidiosis in China.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The United States has experienced a substantial increase in the reported incidence of cryptosporidiosis since 2005. Accompanying this is the emergence of a new subtype of Cryptosporidium hominis based on variation at the 60-kDa glycoprotein (gp60) locus, IaA28R4, which has become a frequently identified subtype in both sporadic and outbreak-related cases. In this study, using multilocus sequence typing (MLST) at eight genetic loci, we characterized 62 specimens of IaA28R4 and 33 specimens of three other gp60 subtypes of C. hominis from four U.S. states with increased cryptosporidiosis incidences during the summer of 2008. Extensive genetic heterogeneity was seen within the gp60 subtype IaA28R4, but specimens from Ohio and southwestern states formed two distinct subpopulations, suggesting that there were at least two origins of IaA28R4 within the United States. Discordance in typing results was observed between gp60 and other genetic markers, especially DZ-HRGP, and this discordance was largely the result of genetic recombination within the gp60 subtype IaA28R4. The results of population genetic analyses supported the presence of two subpopulations of IaA28R4 and the occurrence of genetic recombination within this gp60 subtype. Thus, the IaA28R4 subtype at gp60 is likely a fitness marker for C. hominis, and genetic recombination is potentially a driving force in the emergence of the virulent IaA28R4 subtype in the United States. A rapid evolution of IaA28R4 was indicated by the observation of multiple MLST subtypes of IaA28R4 within two large outbreaks that lasted for extended periods and involved multiple swimming pools.
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