ArticleMolecular human reproduction2014
Human PLCζ exhibits superior fertilization potency over mouse PLCζ in triggering the Ca(2+) oscillations required for mammalian oocyte activation.
Article in Molecular human reproduction, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Pathogenic homozygous PLCZ1 mutation reduces sperm protein levels with a simulated reduction of PIP2 binding: a case report.Reproduction & fertility · 2026Article
- The efficacy of ionomycin - induced artificial oocyte activation in preventing fertilization failure in an ICSI cycle: an eight - year retrospective study.Journal of ovarian research · 2025Article
- A Novel PLCζ Mutation Linked to Male Factor Infertility Induces a Gain-of-Function Effect on CaInternational journal of molecular sciences · 2025Article
- Genomic Insights into Fertilization: Tracing PLCZ1 Orthologs Across Amphibian Lineages.Genome biology and evolution · 2025Article
- The mammalian sperm factor phospholipase C zeta is critical for early embryo division and pregnancy in humans and mice.Human reproduction (Oxford, England) · 2024Article
- High rate of detected variants in maleHuman reproduction open · 2024Article
- Antigen Unmasking Is Required to Clinically Assess Levels and Localisation Patterns of Phospholipase C Zeta in Human Sperm.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Applications of advances in mRNA-based platforms as therapeutics and diagnostics in reproductive technologies.Frontiers in cell and developmental biology · 2023Review
- Molecular regulation of PLCβ signaling.Methods in enzymology · 2023Review
- Diagnosis and Treatment of Male Infertility-Related Fertilization Failure.Journal of clinical medicine · 2020Review
- Perfect date-the review of current research into molecular bases of mammalian fertilization.Journal of assisted reproduction and genetics · 2020Review
- Calcium influx and sperm-evoked calcium responses during oocyte maturation and egg activation.Oncotarget · 2017Review
- WBP2NL/PAWP mRNA and protein expression in sperm cells are not related to semen parameters, fertilization rate, or reproductive outcome.Journal of assisted reproduction and genetics · 2017Article
- Sperm-specific post-acrosomal WW-domain binding protein (PAWP) does not cause Ca2+ release in mouse oocytes.Molecular human reproduction · 2014Article
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9 authors.
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Abstract
A sperm-specific phospholipase C-zeta (PLCζ) is believed to play an essential role in oocyte activation during mammalian fertilization. Sperm PLCζ has been shown to trigger a prolonged series of repetitive Ca(2+) transients or oscillations in oocytes that precede activation. This remarkable intracellular Ca(2+) signalling phenomenon is a distinctive characteristic observed during in vitro fertilization by sperm. Previous studies have notably observed an apparent differential ability of PLCζ from disparate mammalian species to trigger Ca(2+) oscillations in mouse oocytes. However, the molecular basis and confirmation of the apparent PLCζ species difference in activity remains to be provided. In the present study, we provide direct evidence for the superior effectiveness of human PLCζ relative to mouse PLCζ in generating Ca(2+) oscillations in mouse oocytes. In addition, we have designed and constructed a series of human/mouse PLCζ chimeras to enable study of the potential role of discrete PLCζ domains in conferring the enhanced Ca(2+) signalling potency of human PLCζ. Functional analysis of these human/mouse PLCζ domain chimeras suggests a novel role of the EF-hand domain in the species-specific differences in PLCζ activity. Our empirical observations are compatible with a basic mathematical model for the Ca(2+) dependence of generating cytoplasmic Ca(2+) oscillations in mammalian oocytes by sperm PLCζ.
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