ArticleInfection and immunity2014
An Escherichia coli Nissle 1917 missense mutant colonizes the streptomycin-treated mouse intestine better than the wild type but is not a better probiotic.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 42 citations in OpenAlex.
- Metabolic strategies of Enterobacteriaceae gut colonization.Nature reviews. Microbiology · 2026Review
- Genetically engineered probiotic E. coli Nissle 1917 enhances protection against Salmonella via increased adhesion and systemic T-cell responses.NPJ biofilms and microbiomes · 2026Article
- Human milk oligosaccharide mediates mutualism between Escherichia coli and Bifidobacterium bifidum.Nature communications · 2026Article
- An Engineered Variant ofACS synthetic biology · 2026Article
- Article
- Gene-level analysis of core carbohydrate metabolism across the Enterobacteriaceae pan-genome.Communications biology · 2025Article
- Nutrition ofEcoSal Plus · 2024Review
- Peeling the onion: additional layers of regulation in the acid stress response.Journal of bacteriology · 2024Article
- Nitrogen assimilation bymBio · 2024Article
- Sortase-encoding genes,Frontiers in microbiology · 2024Article
- OmpC-Dependent Bile Tolerance Contributes to E. coli Colonization of the Mammalian Intestine.Microbiology spectrum · 2023Article
- Past, Present, and Future of Genome Modification inMicroorganisms · 2022Review
- Local Delivery of Streptomycin in Microcontainers Facilitates Colonization of Streptomycin-Resistant Escherichia coli in the Rat Colon.Applied and environmental microbiology · 2022Article
- ProbioticNutrients · 2021Article
- Characterization of a novel AraC/XylS-regulated family of N-acyltransferases in pathogens of the order Enterobacterales.PLoS pathogens · 2020Article
- Article
- The Role of Butyrate in Attenuating Pathobiont-Induced Hyperinflammation.Immune network · 2020Review
- EnvZ/OmpR Two-Component Signaling: An Archetype System That Can Function Noncanonically.EcoSal Plus · 2020Review
- The LiaFSR and BsrXRS Systems Contribute to Bile Salt Resistance inFrontiers in microbiology · 2019Article
- A SimpleApplied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Previously we reported that the streptomycin-treated mouse intestine selected for two different Escherichia coli MG1655 mutants with improved colonizing ability: nonmotile E. coli MG1655 flhDC deletion mutants that grew 15% faster in vitro in mouse cecal mucus and motile E. coli MG1655 envZ missense mutants that grew slower in vitro in mouse cecal mucus yet were able to cocolonize with the faster-growing flhDC mutants. The E. coli MG1655 envZ gene encodes a histidine kinase that is a member of the envZ-ompR two-component signal transduction system, which regulates outer membrane protein profiles. In the present investigation, the envZP41L gene was transferred from the intestinally selected E. coli MG1655 mutant to E. coli Nissle 1917, a human probiotic strain used to treat gastrointestinal infections. Both the E. coli MG1655 and E. coli Nissle 1917 strains containing envZP41L produced more phosphorylated OmpR than their parents. The E. coli Nissle 1917 strain containing envZP41L also became more resistant to bile salts and colicin V and grew 50% slower in vitro in mucus and 15% to 30% slower on several sugars present in mucus, yet it was a 10-fold better colonizer than E. coli Nissle 1917. However, E. coli Nissle 1917 envZP41L was not better at preventing colonization by enterohemorrhagic E. coli EDL933. The data can be explained according to our "restaurant" hypothesis for commensal E. coli strains, i.e., that they colonize the intestine as sessile members of mixed biofilms, obtaining the sugars they need for growth locally, but compete for sugars with invading E. coli pathogens planktonically.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.