ArticleInfection and immunity2014
Loss of sigma factor RpoN increases intestinal colonization of Vibrio parahaemolyticus in an adult mouse model.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- T6SS1 suppresses pro-inflammatory cytokine transcription to drive immune evasion and systemic infection inInfection and immunity · 2026Article
- RpoN mediates biofilm formation by directly controllingBiofilm · 2025Article
- Prevalence and virulence of Vibrio parahaemolyticus isolated from clinical and environmental samples in Huzhou, China.BMC genomics · 2024Article
- ZrgA contributes to zinc acquisition in Vibrio parahaemolyticus.Virulence · 2023Article
- Transcriptomic Profiles of Vibrio parahaemolyticus During Biofilm Formation.Current microbiology · 2023Article
- L-arabinose affects the growth, biofilm formation, motility, c-di-GMP metabolism, and global gene expression ofJournal of bacteriology · 2023Article
- A Class IV Adenylate Cyclase, CyaB, Is Required for Capsule Polysaccharide Production and Biofilm Formation in Vibrio parahaemolyticus.Applied and environmental microbiology · 2023Article
- Regulatory Small RNA Qrr2 Is Expressed Independently of Sigma Factor-54 and Can Function as the Sole Qrr Small RNA To Control Quorum Sensing in Vibrio parahaemolyticus.Journal of bacteriology · 2022Article
- Biological and transcriptional studies reveal VmeL is involved in motility, biofilm formation and virulence inFrontiers in microbiology · 2022Article
- Characterization of the RpoN regulon reveals the regulation of motility, T6SS2 and metabolism inFrontiers in microbiology · 2022Article
- The Regulatory Functions of σInternational journal of molecular sciences · 2021Review
- Article
- Fis Connects Two Sensory Pathways, Quorum Sensing and Surface Sensing, to Control Motility inFrontiers in microbiology · 2021Article
- Genetic determinants ofBiofilm · 2020Article
- Calcium regulates the mycophagous ability of Burkholderia gladioli strain NGJ1 in a type III secretion system-dependent manner.BMC microbiology · 2020Article
- A GntR Family Transcription Factor (VPA1701) for Swarming Motility and Colonization ofPathogens (Basel, Switzerland) · 2019Article
- Carbohydrate metabolic systems present on genomic islands are lost and gained in Vibrio parahaemolyticus.BMC microbiology · 2019Article
- Rapid biosynthesized AgNPs from Gelidiella acerosa aqueous extract mitigates quorum sensing mediated biofilm formation of Vibrio species-an in vitro and in vivo approach.Environmental science and pollution research international · 2017Article
- Quorum Sensing Regulators Are Required for Metabolic Fitness in Vibrio parahaemolyticus.Infection and immunity · 2017Article
- Bioluminescence Imaging to TrackFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Vibrio parahaemolyticus is the leading cause of bacterial seafood-borne gastroenteritis worldwide, yet little is known about how this pathogen colonizes the human intestine. The alternative sigma factor RpoN/sigma-54 is a global regulator that controls flagellar synthesis, as well as a wide range of nonflagellar genes. We constructed an in-frame deletion mutation in rpoN (VP2670) in V. parahaemolyticus RIMD2210633, a clinical serogroup O3:K6 isolate, and examined the effects in vivo using a streptomycin-treated mouse model of colonization. We confirmed that deletion of rpoN rendered V. parahaemolyticus nonmotile, and it caused reduced biofilm formation and an apparent defect in glutamine synthetase production. In in vivo competition assays between the rpoN mutant and a wild-type RIMD2210633 strain marked with the β-galactosidase gene lacZ (WBWlacZ), the mutant colonized significantly more proficiently. Intestinal persistence competition assays also demonstrated that the rpoN mutant had enhanced fitness and outcompeted WBWlacZ. Mutants defective in the polar flagellum biosynthesis FliAP sigma factor also outcompeted WBWlacZ but not to the same level as the rpoN mutant, which suggested that lack of motility is not the sole cause of the fitness effect. In an in vitro growth competition assay in mouse intestinal mucus, the rpoN mutant also outcompeted the wild type and exhibited faster doubling times when grown in mucus and on individual components of mucus. Genes in the pathways for the catabolism of mucus sugars also had significantly higher expression levels in a ΔrpoN mutant than in the wild type. These data suggest that in V. parahaemolyticus, RpoN plays an important role in carbon utilization regulation, which may significantly affect host colonization.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.