ArticleEuropean archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery2015
Mucosal biofilm detection in chronic otitis media: a study of middle ear biopsies from Greenlandic patients.
Article in European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Differential Susceptibility of Mixed Polymicrobial Biofilms Involving Ocular Coccoid Bacteria (Microorganisms · 2023Article
- Polymicrobial biofilms of ocular bacteria and fungi on ex vivo human corneas.Scientific reports · 2022Article
- Review
- Role of Biofilms in Children with Chronic Adenoiditis and Middle Ear Disease.Journal of clinical medicine · 2019Review
- Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model.Journal of burn care & research : official publication of the American Burn Association · 2019Article
- Middle ear irrigation using a hydrodebrider decreases biofilm surface area in an animal model of otitis media.Laryngoscope investigative otolaryngology · 2018Article
- Nanotechnology-based drug delivery systems for control of microbial biofilms: a review.International journal of nanomedicine · 2018Review
- Recurrent otorrhea in chronic suppurative otitis media: is biofilm the missing link?European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2017Article
- Sub-Optimal Treatment of Bacterial Biofilms.Antibiotics (Basel, Switzerland) · 2016Review
- Current Research Approaches to Target Biofilm Infections.Postdoc journal : a journal of postdoctoral research and postdoctoral affairs · 2015Article
- Strategies for combating bacterial biofilm infections.International journal of oral science · 2015Review
- Micro CT visualization of silver nanoparticles in the middle and inner ear of rat and transportation pathway after transtympanic injection.Journal of nanobiotechnology · 2015Article
- Toxicity of silver nanoparticle in rat ear and BALB/c 3T3 cell line.Journal of nanobiotechnology · 2014Article
- Evaluation of the kinetics and mechanism of action of anti-integration host factor-mediated disruption of bacterial biofilms.Molecular microbiology · 2014Article
- Assessing the Role of Nasal Irrigation in Chronic Otorrhea in Children With Tympanostomy Tubes.Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-facialeArticle
- High Prevalence of Antibiotic-Resistant Otitis Media-Associated Bacterial Flora of Asymptomatic People Living with HIV at Morogoro Hospital, Tanzania.Journal of the International Association of Providers of AIDS CareArticle
- Adenoidal bacterial biofilm in pediatric rhinosinusitis.Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologieArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objectives of this study were to examine middle ear biopsies from Greenlandic patients with chronic otitis media (COM) for the presence of mucosal biofilms and the bacteria within the biofilms. Thirty-five middle ear biopsies were obtained from 32 Greenlandic COM patients admitted to ear surgery. All biopsies were examined by means of peptide nucleic acid-fluorescent in situ hybridization (PNA-FISH), and if possible culture and polymerase chain reaction (PCR) of the 16s rDNA and sequencing. Light microscopy and confocal laser scanning microscopy were used. Skin biopsies from 23 of the patients served as controls. PNA-FISH showed morphological signs of biofilms in 15 out of 35 (43 %) middle ear biopsies. In the control skin biopsies, there were signs of biofilms in eight out of 23 biopsies (30 %), probably representing skin flora. PCR and 16s sequencing detected bacteria in seven out of 20 (35 %) usable middle ear biopsies, and in two out of ten (20 %) usable control samples. There was no association between biofilm findings and PCR and 16s sequencing. Staphylococci were the most common bacteria in bacterial culture. We found evidence of bacterial biofilms in 43 % of middle ear biopsies from patients COM. The findings may indicate that biofilms are a part of the pathogenesis in recurrent episodes of ear discharge in COM, but further investigations are necessary.
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